Thermo Finnigan LTQ-FT mass spectrometer- LC/MS/MS complete with Edwards E2M30
pumps, Pfeiffer Duo 25 pump, Pentium IV PC with Windows XP Professional, HP
Laserjet Printer 2300d, Dell 19" LCD monitor, Xcalibur 2.0 software with
license and manuals.
The Thermo Finnigan LTQ FT hybrid linear ion trap - Fourier Transform ICR
mass spectrometer provides high resolution, accurate mass determinations, and
MS-n. The FT Ion Cyclotron Resonance analyzer has an actively shielded 7 Tesla
superconducting magnet. Ions trapped in the ICR cell are excited by applying
a SWIFT (Stored Waveform Inverse Fourier Transform) waveform. Mass range is
50 to 2000 in FT mode. The FT resolution is user selectable from 12,500 (FWHM)
up to a maximium mass resolution of 500,000 at m/z 400.
It is capable of simutaneous high resolution, mass accuracy, and sensitivity
over one mass decade (e.g. m/z 200 - m/z 2000). It has a fast data acquisition
rate (1 second repetition rate) with very high resolution (100,000 at m/z 400).
The mass accuracy is better than 2 ppm with external calibration. Sensitivity
is sub fmol on-column in LC/MS. It is cabable of simultaeous full scan FT MS,
data dependent zoom scan, and MS/MS scans. It is equipped with several nanoESI
sources (including static, dynamic, and packed tip) with a Pulnix video camera
and has the standard Finnigan IonMax API source with the ESI attachment. The
ICR cell has an Electron Capture Dissociation (ECD) emitter cathode attached
to the back of the cell. The ECD method of fragmentation produces c and z ions.
This method preserves co- and post-translational modifications (PTMs) such as
phosporalation, O- and N-linked glycosalation and sulfation. The LTQ also has
Wide Band Activation which allows the simultaneous fragmentation of a parent
ion and its corresponding water loss peak by resonance excitation.
SPECIFICATIONS
- Resolution to 100000
- Mass Accuracy 1-2 ppm with internal standard
- Mass Accuracy <1 ppm Sim mode
- Electron Capture Dissociation Capable
APPLICATIONS
- High accuracy molecular weight determination
- Top down protein microsequencing and identification
- Bottom up protein microsequencing and identification
Please contact us for complete LTQ-FT system, warranty and service contract
details.
