The Spero developed by Daylight Solutions is a purpose-built fully integrated high spatial resolution high throughput Mid Infrared rapid imaging microscopy system. Spero incorporates Quantum Cascade Laser technologies and provides full frame, real-time imaging capabilities previously unavailable with benchtop instrumentation.
Key Features
Some of the key features of Spero include::
- Real-time chemical imaging
- Rapid acquisition of full-spectrum hyperspectral datacubes
- High-brightness, broadly tunable laser source
- Synchrotron class spatial resolution and sensitivity
- Specifically designed for mid-infrared spectral imaging analysis of materials research and biomedical samples
- Reflection and transmission modes of operation
- Fully automated stage
- Visible and infrared high N.A objectives
- Instrument control and data display via Ethernet to PC
- Turn-key small footprint integrated mid Infrared chemical imaging incorporating room temperature focal plane array
3D Infrared Microscopy for Preclinical Pathology
Specifications
The specifications of Spero are given in the table below:
| SPECIFICATION |
MODE of OPERATION |
| Parameter |
Sym |
Units |
Hi-Mag |
Lo-Mag |
Visible |
| Camera Array Size |
-- |
-- |
480 x 480 |
480 x 480 |
3k x 2k |
| Image Pixel Size |
-- |
µm |
1.4 µm |
4.3 µm |
0.9 µm |
| Diffraction-Limited Spatial Resolution |
Δx |
µm |
< 5 µm @ v = 1800 cm-1 |
< 25 µm @ v = 1800 cm-1 |
< 3 µm @ λ = 0.55 µm |
| Numerical Aperture |
NA |
-- |
0.70 |
0.15 |
0.13 |
| Wavelength Coverage |
Δλ |
µm (cm-1) |
5.5 µm – 11.1 µm (900 cm-1 - 1800 cm-1) |
0.45 µm - 0.65 µm |
| Spectral Resolution |
Δν |
cm-1 |
4 cm-1 |
N/A |
| Magnification |
M |
- |
12.5X |
4X |
4X |
| Working Distance |
WD |
mm |
> 5 mm |
> 25 mm |
> 15 mm |
| Field of View |
FOV |
mm |
650 um |
2000 um |
650 um |
| Frame Rate |
-- |
fps |
30 fps |
30 fps |
3D Histology with the Spero™ Infrared Microscope