An additional fluorescence detector is included in the SX/DF option to allow concurrent fluorescence detection at two emission wavelengths. The two detectors are mounted directly onto the cell-block. A cut-off filter is generally used with one detector in order to isolate emission at the higher wavelength, and an interference filter is used with the second detector to separate emission at the lower wavelength. The SX/DF accessory is often used in the study of FRET reactions allowing simultaneous recording of both donor and acceptor kinetics. The SX/DF option needs a dual detection channel with the SX20 or SX20-LED system.
SX/FP Fluorescence Polarization/Anisotropy
When a fluorophore is excited with plane polarized light, it leads to the preferential excitation of molecules with their absorption moments orientated parallel to the polarization plane. Fluorescence polarization/anisotropy can give data regarding changes in a fluorophore’s mobility and environment when it interacts with other molecules.
The SX/FP fluorescence polarization/anisotropy accessory is a dual channel T-format fluorescence polarimeter that can be fitted easily. The software is used to control g-factor determination and both spectra and kinetics may be needed in anisotropy, polarization, raw data (∥ and ⊥), and total emission modes, and with full post-acquisition conversion between data modes as needed. Option SX/DF is included in option SX/FP as described above and similarly needs a dual detection channel with the basic SX20 or SX20-LED system.
SX/APSSH01 Steady-State Sample Housing
The steady state sample housing allows temperature-controlled UV-Vis or fluorometric measurements using a regular cuvette – using monochromator(s) and the SX20 light source. It is fixed to the monochromator either directly or through the standard fiber optic light guide and connected to either the PDA detectors or the absorbance/fluorescence PMT detectors.
This information has been sourced, reviewed and adapted from materials provided by Applied Photophysics.
For more information on this source, please visit Applied Photophysics.