Rapid, All-Elemental BioImaging

The combination of the icpTOF and rapid laser ablation systems enables all-element imaging at much faster speeds than conventional LA-ICP-MS instrumentation – enabling practical routine imaging of large areas of tissue.

Olga Borovinskaya, Oliver-Bolle Bauer, Uwe Karst
TOFWERK, The University of Muenster, Teledyne CETAC Technologies

Pt-Based Compounds

In spite of substantial advances to anti-cancer drug development, Pt-based compounds continue to represent the most regularly utilized anti-tumor agents for chemotherapy. Comprehension of drug distribution, accumulation and metabolism is a necessary precondition for optimizing Pt-based treatments and minimizing side-effects.

Laser Ablation ICP-MS Imaging

As a result of the methodology’s low detection limits and measurement capacities, laser ablation (LA) ICP-MS imaging of Pt in tissue has acquired substantial recognition over the last ten years.

However, standard implementation of the methodology is restricted by the considerable time needed for analysis. For example, the standard instrumentation requires nearly 30 hours to image a 5 mm2 area with lateral resolution of 15 μm (e.g., JAAS, 201 2, 27, 1 59- 1 64).

After they have been coupled, the icpTOF and recently developed rapid laser ablation systems substantially improve imaging speed without undermining lateral resolution or sensitivity. Image blurring is decreased by synchronizing the mass spectrometer and laser on a single-laser-pulse basis. This research demonstrates an instance of Pt-imaging in a thin section of a rat kidney that was perfused with Cisplatin, a Pt-containing chemotherapy medication.

In 5 hours, a 10 x 7 mm tissue section was imaged, with every element documented. Higher Pt concentrations were observed in the cortex region in comparison to the medulla and pelvis, as a result of the occurrence of the proximale tubuli cells in this region. Maps of other elements, such as Cu, may yield further sample information.

Cu intensity map (left) and Pt concentration map (right) of 5-µm thin section of a rat kidney perfused with Cisplatin. Ablation was performed at 20 Hz laser frequency, with a 20 µm square laser spot size, 2 J/cm2 fluence and scan speed of 200 µm/s. At this scan speed 10 laser pulses were applied to every 20 µm spot and 10 signals of single laser pulses were averaged to produce the signal of one pixel. Pt was quantified using external calibration with a series of in-house (University of Mu¨nster) produced element standards embedded in Technovit.

Cu intensity map (left) and Pt concentration map (right) of 5-μm thin section of a rat kidney perfused with Cisplatin. Ablation was performed at 20 Hz laser frequency, with a 20 μm square laser spot size, 2 J/cm2 fluence and scan speed of 200 μm/s. At this scan speed 10 laser pulses were applied to every 20 μm spot and 10 signals of single laser pulses were averaged to produce the signal of one pixel. Pt was quantified using external calibration with a series of in-house (University of Münster) produced element standards embedded in Technovit.

This information has been sourced, reviewed and adapted from materials provided by TOFWERK.

For more information on this source, please visit TOFWERK.

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