FluoroMax-4 Compact Spectrofluorometer from HORIBA

HORIBA has launched a new, compact spectrofluorometer called FluoroMax-4. This tool delivers the required sensitivity essential in fluorescence analysis and includes features which are not available in majority of table-top fluorescence detection systems. When compared to its predecessor, FluoroMax-3, the FluoroMax-4 provides 20% better signal-to-noise ratio and 33% higher sensitivity.

Additionally, FluoroMax-4P is available from HORIBA which can be used to upgrade TCSPC for fluorescence lifetimes or perform phosphorescence measurements.

A xenon source in the spectrofluorometer delivers superior UV performance. The bulb is mounted vertically because mounting the bulb in a horizontal fashion can make the arc to sag which can cause instability and reduce the life of the product.

Using an elliptical mirror, the xenon source is made to focus on the entrance slit of the excitation monochromator. The reflective surface ensures efficient collection and also ensures that all the wavelengths remain focused on the slit. This is in contrast to lenses having chromatic aberrations, making them effective for just one wavelength.

A supplementary xenon flash lamp, which is software-selectable, can be fixed to the spectrofluorometer to allow phosphorescence studies.

Key Features

Product features of the FluoroMax-4 Compact Spectrofluorometer are:

  • The slits: They can be constantly and bilaterally modified from the computer in units of millimeters or bandpass. This helps in maintaining optimum resolution and immediate reproducibility
  • The Reference Detector: Just before the excitation light arrives at the sample, a photodiode reference detector checks the intensity as a function of wavelength and time. When compared to conventional rhodamine-B quantum counters, the photodiode detector has better wavelength response and does need any maintenance.
  • The Excitation Monochromator: The aspheric design of the excitation monochromator makes certain that the light’s image diffracted by the grating fits within the slit. The plane, blazed gratings eliminate two main drawbacks common in concave holographic gratings which are insufficient imaging during scans which tend to scatter light and poor polarization performance. Additionally, the ‘wavelength drive’ scans the grating at 80 nm/s speed, and the grating grooves are then blazed to deliver optimum light in the visible region and UV.
  • The Sample Chamber: In order to promote the use of accessories for special samples, a large sample chamber is provided. This chamber also allows users to adopt different sample schemes.
  • The Emission Detector: Photon-counting is utilized by emission detector electronics to provide the ultimate in low-light-level detection. Also, photon-counting focuses on signals that arise from fluorescence emission and ignores smaller signals generated in the detector tube (PMT). The usual technique of analog detection employed by conventional fluorometers combines both signal and noise which tend to camouflage weak emissions.
  • The Emission Monochromator: The entire set of exceptional features of the excitation monochromator is integrated within the emission monochromator. In addition, gratings are blazed to deliver higher throughput in the visible region.
  • The emission detector housing includes a high-voltage supply to offer signal-to-noise ratio.

Specifications

The specifications of the FluoroMax-4 Compact Spectrofluorometer are provided in the table below:

Wavelength Range 200–950 nm
Optical System Plane-grating Czery-Turner Spectrometer
Detector(s) Photomultiplier
Light Source Ozone-free Xenon Lamp
Scanning Speed up to 80 nm/second
Wavelength Accuracy +/- 0.5 nm

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