The Zetasizer µV adds the power of advanced light scattering techniques to your laboratory to improve accuracy, reliability and ease of use for the characterization of protein solutions.
- Dynamic light scattering enables the accurate determination of the molecule size and the derived molecular weight. It also allows further characterization of the protein size distribution to determine the presence and proportion of aggregates.
- Static light scattering enables you to determine the absolute molecular weight and 2nd virial coefficient of your protein in the sample buffer - a value indicating how well your protein is likely to stay in solution.
The new technology incorporated in the Zetasizer µV series delivers unequalled sensitivity and versatility. The Single Mode Fiber (SMF) technology takes protein measurements to new levels of sensitivity with the possibility of detecting down to a hydrodynamic radius of 0.3 nm. The performance capabilities of the Zetasizer Î¼V can only be fully appreciated with the dedicated advanced software.
We use the Zetasizer µV to characterize the size of proteins and how they interact to produce multimeric forms or even supramolecular arrangements of the protein such as viral capsids [shells], The Zetasizer µV allows us to work with sizes in the range of 1 nm to 500 nm and follow each assembly and stability parameter, such as pH, temperature and ionic strength, guiding us to the optimal production conditions for these virus-like particles.
Dr Ismael Bustos- Jaimes,Laboratory of Physical Chemistry and Protein Engineering, UNAM