The Zetasizer Nano ZS is a high performance, two angle particle and molecular size analyzer for the improved detection of aggregates and measurement of small or dilute samples. It also measures samples at very low or high concentration using dynamic light scattering with ‘NIBS’ optics.
The ZSP also includes a zeta potential analyzer that uses electrophoretic light scattering for particles, molecules and surfaces, and a molecular weight analyzer that uses static light scattering.
Furthermore, a microrheology option is available for measuring sample viscosity and viscoelastic properties, as well as a Protein Measurement option for protein mobility measurements. The flow mode option enables the system to be connected to an SEC or an FFF system to use as a detector for the size of proteins or nanoparticles.
A choice of cuvettes are available, from disposable single-use to specific cells for viscous or high concentration samples for measuring the zeta potential of surfaces.
The key features of the Zetasizer Nano ZS are:
- Size measurement from 0.3nm (diameter) to 10µm using patented NIBS (Non-Invasive Back Scatter) technology.
- Zeta potential using patented M3-PALS technology.
- Molecular weight measurement down to 980Da.
- Microrheology option to measure viscosity and viscoelasticity.
- Protein size measurement as dilute as 0.1mg/mL (Lysozyme).
- Sample concentrations from 0.1ppm to 40%w/v.
- Optional protein calculators, including protein charge, A2, kD, and molecular shape.
- A ‘Quality Factor’ and ‘Expert Advice System’ gives the confidence of having an expert at your shoulder.
- 21CFR part 11 software option to enable compliance with ER/ES.
- Research software option to give access to further features and analysis algorithms for the light scattering specialist.
- Automation of measurements using an autotitrator option.
- Optional flow mode capability to enable use as a GPC / SEC size detector.
- Alternative laser, 50mW at 532nm for samples incompatible with the standard 633nm laser.
- Optical filter option to improve measurements with fluorescent sample.
- Temperature range extension option to 120ºC.
Being able to measure particle size is important for the determination of the hydrodynamic radius of the particles including the polymer shell in comparison to other methods like TEM, visualizing the particle radius without polymer shell.
Dr. Sabine Kosmella, University of Potsdam