Polymer or protein concentration profile need to be accurately measured in order to obtain high-quality molecular weight or structure data. The high-stability Viscotek UV detector is suitable for multi-detection GPC/SEC. It is sold as a stand-alone module for connecting other chromatography components, including the 270max or the SEC-MALS 20, or as an additional component to the Viscotek TDAmax for completion of a Tetra Detector format.
The mobile phase flow through a UV detector flow cell is enabled by a pump, for instance the GPCmax, and some type of injection device, for instance a manually actuated valve or autosampler. This flow cell is placed inside the detector array housing itself in the case of the TDAmax system. Fiber optic cables are used to transmit the light from the UV detector through the flow cell.
The response provided by the compounds with a UV chromophore on the detector varies in proportion to the concentration. The absorbance is measured by the UV detector at the user-specified wavelength. The absorbance data is recorded by the OmniSEC software as a chromatogram, in conjunction with all the other data channels. This data is then applied for estimating concentration or conjugate data based on the analysis settings in the software.
Key Features and Benefits
The following are the key features and benefits of the Viscotek UV detector:
- Superior data reproducibility and baseline stability
- Ideal instrument for performing the compositional analysis of protein conjugates or co-polymers
- With a wavelength range of 190-500nm, the most appropriate wavelength can be selected based on the sample of interest
- Wavelength accuracy is ±3nm
- The detector cell and the rest of the system are placed in the same thermal chamber, thus enabling precise and accurate temperature control
We rely heavily on the Malvern Panalytical HT-GPC system because of the comprehensive, accurate information it provides. GPC analysis of our polymers is challenging but the system performs consistently and reliably. Ongoing costs are low because the system is easy to maintain and consumables are affordable, which drives down the ‘cost per injection’, an important comparative indicator for us. And the software is excellent. It gives us the flexibility we need to fully explore our materials and at the same time allows new users to get useful data with minimal training.
Dr Jerome Claverie, Director, NanoQAM