Automated Ultra-Low Volume Taylor Dispersion Analysis – Viscosizer TD

Malvern Panalytical Viscosizer TD is an automated tool that employs Taylor Dispersion Analysis for biophysical characterization and offers ultra-low volume, solution-based molecular size and stability measurement in addition to relative viscosity assessment using Poiseuille flow. It offers an orthogonal technique for conformational stability analysis, which can be extended for highly complex solutions right from small molecules through to protein-based systems.

With Taylor Dispersion Analysis having UV area imaging in microcapillary flow, Viscosizer TD allows ultra-low volume biophysical characterization of target biomolecules in solution. Taylor Dispersion Analysis is an experimental method in which a laminar flow of matched buffer is subjected to a nanoliter volume sample pulse.

The hydrodynamic radius of solute molecules and the molecular diffusion coefficient can be determined by analyzing the time-evolved concentration profile (or Taylorgram) from UV absorption.

The fully automated concept of the Viscosizer TD enables analysis of the stability of proteins, peptides, small molecules and a mixture of all these, even in the presence of surfactants and excipients, which can interfere results in other measurement techniques. Poiseuille’s law-based microcapillary flow method enables complementary relative viscosity measurement on the same sample.

Key Features

The main features of the Viscosizer TD are:

  • Easy to use
  • Allows optimization of measurement sensitivity and molecular selectivity by changing UV wavelength
  • Orthogonal technique for protein stability assay and protein solubility studies
  • Taylor Dispersion Analysis enables characterization of unmatched molecular size range in solution, from small molecule size, peptide size and protein size, and samples with mixtures of these species
  • Matched sample buffer renders excipients and surfactants invisible, enabling label-free characterization of target
  • High resolution measurements with high reproducibility at low concentration and low viscosity
  • UV detection for molecular sizing allows low concentration measurements
  • Total sample volume of 35µL required for triplicate measurements for both size and relative viscosity

Applications

The major applications of the Viscosizer TD are:

  • Assessment of Critical Quality Attributes (CQA) for biotherapeutics
  • Optimization of lead candidates in complex buffers during formulation development
  • Conducting biophysical studies of peptide-based therapeutics at low concentration
  • Advancing understanding of drug behavior at near in vivo conditions
  • Screening for developability of mAbs at early stages

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