Two years after the acquisition of PTI, HORIBA has now launched the PTI QuantaMaster™ 8000 series of fluorometers. These new modular research fluorometers provide the highest guaranteed sensitivity specification in the world, along with several other unique benefits. The only product that matches the sensitivity of the QuantaMaster 8000 series is the HORIBA Fluorolong 3, which is also a product of HORIBA Scientific.
- It guarantees the highest sensitivity specification at a ratio of 30,000:1 Water Raman RMS
- Makes use of world class software for all steady state and lifetime requirements
- Extended wavelength range with triple grating monochromators
- World class TCSPC lifetime enhancements
- Completely automated system
- World class stray light rejection with large single or double additive, coma corrected, monochromators
- Efficient flexibility with up to four light sources and six detectors that can be selected
- NIR steady state and phosphorescence lifetime detection to 5,500 nm
Lifetime Measurements TCSPC
The TCSPC fluorescence lifetime capabilities can be used to enhance the QuantaMaster series. The ultimate speed, performance and versatility are provided by the QuantaMaster, using TCSPC sources, detectors and electronics created by the IBH group. To perform additional TCSPC measurements, the standard Quantamaster PMT can be used or one can append a dedicated TCSPC detector with an upgraded performance or the extended NIR detection. The FeliXGX software deals with the entire steady state and time-resolved control, attainment, and analysis.
HORIBA TCSPC Benefits
- The company has 40 years of experience in TCSPC innovation
- TCSPC lifetime measurements from under 25 ps
- Industry-leading true 100 MHz system operation enables millisecond acquisition times
- Measure TCSPC lifetimes, time-resolved anisotropy and TRES
- Catalog consists of more than 60 latest compact pulsed LEDs and Laser Diodes for almost any kind of application
- Complete control over TCSPC and steady state acquisitions with single Felix GX software package
- PowerFit-10 decay analysis package with multiple fitting models including a novel Maximum Entropy Method (MEM) lifetime distribution program
- For efficient versatility select a picosecond Supercontinuum Laser with HORIBA’s proprietary Frequency Doubler – a strong tool for Time-Resolved protein studies
Two Systems can be Better Than One!
The company offers stand-alone TCSPC at an affordable price. By having a dedicated TCSPC system and a dedicated steady state fluorometer operating at the same time, the throughput of the lab can be increased. This is offered for almost the same price as adding the TCSPC to the QuantaMaster.
Near-infrared (NIR) spectrofluorometry has developed as a significant analytical method, particularly in the fields of nanotechnology, material research, photomedicine and chemistry. The PTI offers strong and diverse NIR capabilities, either as an upgraded version of its UV-VIS steady state spectofluorometers or as a research level fluorometer that can be used on its own. There exist different configurations that can be accustomed to serve several research needs.
NIR-PMT Based Steady-State Systems
The system consists of a scanning monochromatos, high intensity continuous xenon light source, and a cooled NIR PMT detector. There are four NIR PMTs available that provide maximum spectral range coverage. The spectral range coverage includes:
- 300 nm–1,400 nm, LN-cooled
- 950 nm–1,400 nm, TE-cooled
- 300 nm–1,700 nm, LN-cooled
- 950 nm–1,700 nm, TE-cooled
Solid-State Photodiode Based NIR Steady State Systems
The system comprises of a scanning monochromators, high intensity continuous xenon light source, lock-in amplifier and chopper for noise suppression. There exists a broad range of photo diodes, TE-cooled or LN-cooled up to 2400 nm:
- InGaAs: 500 nm–1,700 nm (up to 2400 nm detector available upon request)
- PbS: 1,000 nm–3,200 nm
- InSb: 1,500 nm–5,500 nm
Additions for NIR Lifetime Measurements to 5,500 nm!
To measure the lifetime from 1 µs to hundreds of ms, the above listed detectors can be employed in a single shot transient digitizer (SSTD) mode for the luminescence lifetime measurement capability in NIR. The SSTD is exceptionally rapid and provides excellent signal to noise, add:
- Variable high rep rate pulsed xenon lamp option for phosphorescence lifetime (NIR-TR-10)
- 3rd party pulsed Q-switched lasers
- Pulsed nitrogen and dye laser (NIR-TR-20)
- TCSPC lifetime add-on with supercontinuum laser, laser diodes and LEDs (for NIR-PMT based systems)
Unique NIR Solutions
To cover up the broad range of spectra for both fluorescence and phosphorescence lifetimes and for steady state spectra, the PTI QuantaMaster can be supplied with various detectors and illuminators. The following configuration is to be considered:
- Double emission monochromator with R928 PMT, InGaAs &, InSb detectors cover 250 to 5,500 nm
- 20 Hz Q-switched/OPO Opolette laser for tunable excitation from 210 to 2,200 nm
- Or frequency doubled nitrogen pumped dye laser for tunable excitation from 250 to 990 nm
- Continuous xenon lamp for steady state spectra
- Single Shot Transient Digitizer (SSTD) phosphorescence decays over entire range from 250 to 5.500 nm
- Steady state spectra from 250 to 5,500 nm
Modularity to Grow
In order to be accustomed to the needs of the future fluorescence applications, an open architecture design is featured by the PTI QuantaMaster series, which eventually provides the versatility. By selecting the PMT tubes, the light source, gratings and also a wide variety of existing accessories, the initial configuration can be optimized. The available number of configurations is essentially limitless.
The spacious design of the PTI QuantaMaster universal QuadraCentric sample compartment can hold a broad selection of sample accessories and provides accessibility. Users can select from sample temperature controllers to different holders for liquids, solids and powders and several other options.
The methodology and application changes are also allowed by the open architecture design. The PTI QuantaMaster grows based on the needs of the application. For example, if the user wants to measure dynamic anisotropy, they can add a set of polarizers and a second emission channel.
To compliment steady state data with lifetime measurements, users can add a laser or LED-based excitation to the initial configuration. For those interested in intracellular Ca2+, after finishing initial Fura-2 studies, individuals can decide when to start imaging the events. The coupling of the system with any kind of fluorescence microscope is simple. The possible configurations that can be made are limitless even if the user wants to add a second excitation source or NIR detection.
Applications and Examples - UV VIS Fluorescence Spectroscopy
- Ratiometric measurements for intracellular ions
- Synchronous Fluorescence Spectroscopy (SFS)
- Time based measurements
- Total Luminescence Spectroscopy (TLS)
- Quantum yield
- Automated temperature control
- Phosphorescence with a pulsed light source
- Förster Resonance Energy Transfer (FRET)
- Electroluminescence and photovoltaic measurements
- Steady state anisotropy
Applications and Examples: NIR Fluorescence Spectroscopy
- Materials Science
- Glasses and ceramics
- Lasing media and LEDs
- Upconverting nanoparticles for tissue imaging
- Photobiology and photomedicine
- Singlet oxygen detection
- R&D of singlet oxygen photosensitizers
- Photodynamic Therapy (PDT)
- Optical fiber communication
- Optical amplifiers (e.g. chelated Er3+, 1540 nm)
- NIR luminescence of minerals
- Photo-oxidation of environmental pollutants
- Forensic science
- Detecting forged documents
- Photoluminescene of Lanthanides
- Optical fiber communications
- Single walled carbon nanotubes
- Singlet oxygen detection