High-Resolution, Automated Inverted Microscope - IX83

The fully motorized IX83 from Evident is the most advanced microscope in the IX3 series of inverted imaging systems.

This advanced microscope offers a new level of application flexibility. Select from the one-deck system with a low, ergonomic stage or the two-deck system with extra expansion capabilities. Both offer the ability to perform a multitude of different imaging applications, from long-term time-lapse imaging and other challenging methods, to regular testing and documentation.

Users can customise the parts and controls to suit their needs.

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Expandable to Meet Growing Research Needs

The fully-motorized IX83 is capable of satisfying a wide range of research needs. With extra modules offering expanded functionality, both microscope options enable different imaging methods, ranging from casual documentation to long-term time-lapse imaging and other demanding methods.

The IX83’s unique open frame provides easy access to the light path, making it easy to incorporate or change modules. Several deck modules can be easily exchanged to add or remove functions as required.

The IX3-ZDC2 Z drift compensator module with its simple slide-in design can be easily integrated into any IX83 system to maintain continuous focus throughout an extensive time-lapse experiment.

  • IX83: Two-deck System - Enables high-speed, fully automated device selection during advanced image acquisition and live cell research. The two-deck configuration offers superior expandability.
  • IX83: One-deck System - An intelligent, motorized microscope has a large field number (FN22, left side port) and IX3-ZDC2 compatibility, thus forming a new standard for live cell imaging.

Reliable, Clear and Bright High-Resolution Images

Evident UIS2 infinity-corrected optics guarantee high optical transmittance with a wide range of objectives.

The UIS2 optics feature broad chromatic correction and enable high resolution, high S/N primary images irrespective of the observation technique. The broad field of view and Fly-Eye lens system delivers uniform fluorescence images and allow the use of sCMOS cameras with large sensors.

Excellent Image Quality

Apochromatic Objectives Enable High-Resolution Phase Contrast and Fluorescence Observation

Phase contrast apochromatic objectives (UPLSAPO100XOPH, PLAPON60XOPH) allow high-precision imaging devoid of image shift, even during concurrent phase contrast and fluorescence observation. This eliminates the need to alter objectives when switching observation techniques.

Silicone Objectives* Enable High-Resolution Observation Deep into Live Cells

Evident offers three high-NA silicone immersion objectives: UPLSAPO30XS, UPLSAPO40XS, and UPLSAPO60XS. The refractive index of silicone oil (Refractive index: ne≈1.40) is near that of living tissue (Refractive index: ne≈1.38), enabling high-resolution observation deep within living tissue with minimum spherical aberration caused by refractive index disparity.

Silicone oil does not harden or dry out, so there is no need to re-administer the oil, making it suitable for prolonged time-lapse observations.

Special Objective Available for iPS/ES and Floating Cell Observation

This high-NA phase contrast objective (UCPLFLN20XPH) is particularly suited for observation using plastic dishes. It enables high-resolution observation of the cell proliferation process and supplies enhanced contrast across a broad area.

Bright, Uniform Fluorescence Illumination

The fluorescence illuminator (IX3-RFALFE) is designed with a Fly-Eye lens system to provide uniform light distribution. This provides bright and uniform illumination to the whole field, including the periphery of the visual field.

High S/N Fluorescence Mirror Units for Efficient Signal Detection

All fluorescence mirror units feature filters treated with a specially-developed coating that decreases noise by absorbing over 99% of stray light. The exceptional performance and high transmittance of the mirror units guarantee efficient fluorescence signal detection.

*Use dedicated silicone oil.

Intuitive and Ergonomic Microscope Control

Smart Control

  • Switch Observation Methods with a Single Touch: A touch panel controller is offered by Evident along with the IX83, which allows the user to easily configure all automated operations on the microscope. This includes advanced operations such as magnification-specific lamp intensity adjustment. The touch panel controller, used along with cellSens software, enables advanced customization of the system.
  • ZDC One-Shot Function Quickly Detects Focus, Even during High-Magnification Observation: IX3-ZDC2 focus detection and tracking can be done using the innovative touch panel controller, without the need of software. There is also a focus search task supported by a cell-safe, near-infrared laser, allowing users to instantaneously focus on samples, even at high magnification.
  • Intuitive Microscope and XY Stage Controllers: The U-MCZ focus module and XY stage controller offer the ease of conventional handle operation for smart microscope control, even in a darkroom environment.
  • Stored Microscope Configurations (Evident cellSens): The system saves microscope configurations together with image data by integrating a readout of motorized and coded unit position. With this advanced system, a broad range of settings can be recalled to restructure the desired imaging conditions, thus developing a highly reproducible and user-friendly, high-end imaging system.

Operator-Friendly Design

  • Reliable Tracking at High Magnification: The IX3-SVR manual stage features a reliable positioning system which enables the easy tracking of cells, even at high magnifications. The travel limits can be controlled by the user to halt the stage and prevent unplanned movement during vital operations. It is also possible to remove the 35 mm dishes from the stage, keep them in an incubator, and return them to the exact location previously identified.
  • Easily Achieve Koehler Illumination: The condenser can be moved and easily reset to Koehler Illumination using control knobs and the conveniently-located condenser lock.
  • Catch Tray Minimizes Optical System Contamination: A catch tray under the nosepiece reduces the likelihood of contaminating and damaging the microscope with spilled liquids. This feature also makes maintenance simple.

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Ideally Suited for Live Cell Imaging

With the IX83, dynamic cellular processes can be easily captured. This imaging system is designed for outstanding time-lapse imaging.

Its new frame architecture and focus drive design offers improved rigidity that decreases the impact of vibration and changes in temperature. It maintains desired positions along the X, Y, and Z axes to allow reliable time-lapse and multipoint imaging.

When integrated with the Evident IX3-SSU ultrasonic stage and Z drift compensator (IX3-ZDC2), the IX83 is ideally suited for capturing high-precision, multipoint time-lapse images that are never misaligned or out of focus. Box-type and stage-top incubators are also available to maintain the practicality of live cells during time-lapse experimentation.

Imaging Accuracy

  • Z Drift Compensation System (IX3-ZDC2): The new, fully motorized Evident Z drift compensation module overcomes focal drift to maintain the sample in sharp focus during long-term, time-lapse experiments. The enhanced IX3-ZDC2 is faster, more flexible, and supports a larger range of objectives and sample vessels.
    A one-shot autofocus (AF) mode allows a number of focus positions to be set as desired for thicker samples, enabling efficient Z-stack acquisition in multi-position experiments. The improved continuous AF mode, at present also supporting dry objectives, maintains the desired plane of observation precisely in focus, even during changes in room temperature and the addition of reagents.
  • High-Precision Ultrasonic Stage for IX3 (IX3-SSU): With low thermal drift and high accuracy, the ultrasonic stage offers better-quality reliability for multi-image acquisition. Sample holders tightly secure dishes or slides to guarantee accurate repeatability during high-magnification, multi-point observations.
  • Efficient Multi-Image Acquisition with cellSens Software: The IX3 ultrasonic stage can be used with cellSens software to perform multi-area time-lapse imaging with better efficiency. Image cell dynamics at numerous points within each well and join adjoining fields to form wide area, time-lapse images and acquire a greater quantity of data from the same experiment. The outstanding repeatability and accuracy of the ultrasonic stage makes it easy to achieve seamless tiled images with cellSens software.
  • Motorized DIC Slider for IX83: The motorized IX3-DICTA allows users to engage and alter DIC without causing any disturbance to the sample.

Interchangeable Modules Provide Flexible Imaging Options

A broad range of units are available for the Evident IX3 microscope system, bringing improved functionality to casual observation all the way to high-end imaging. Uncomplicated cassette-like insertion into the deck makes it simple to mount right side ports, fluorescence mirror turrets, epi-illuminators, mag changers, and other units.

The large open frame allows a motorized emission filter wheel to be installed within the microscope’s infinity space. This removes image shift between channels and allows the eyepieces to observe what the camera detects.

Deck Units/High Speed Units

  • Motorized Fluorescence Mirror Turret (IX3-RFACA): A non-click turret that can be installed with up to 8 mirror units and that delivers smooth, quick cube rotation. The turret can house both 25 mm and 32 mm diameter filter units, which can easily be inserted and taken out without the requirement of any tools.
  • Motorized/Manual Right Side Port with C-mount (IX3-RSPCA/IX3-RSPC): The C-mount-equipped right side ports can be installed with up to two mirror units, allowing advanced applications such as split imaging.
  • Coded Intermediate Magnification Changer (IX3-CAS): Magnification can be easily modified between 1X, 1.6X and 2X by altering the lever. As the system is coded, information on intermediate magnifications is saved with image data.
  • Motorized Fast Filter Wheels and Shutters: The user can easily switch between filters in milliseconds using the Evident filter wheels. Evident shutters function even faster. The IX83 can control up to six filter wheels and four shutters, thereby enabling intricate, multi-modal imaging.

Fluorescence System

  • Application-Specific Fluorescence Illuminators: There is an illuminator well-suited for each application. The L-shaped fluorescence illuminator with the Fly-Eye lens system (IX3-RFALFE) offers bright, uniform illumination without adjustment.
    The standard L-shaped fluorescence illuminator (IX3-RFAL) is cost-effective and is fitted with both field and aperture iris diaphragms. The straight fluorescence illuminator (IX3-RFA) is perfect for applications requiring strong excitation light. A broad range of light sources is also provided, including counter frame and top-mounted units (xenon and mercury).
  • Long Life 130W Mercury Lamp (U-HGLGPS): The U-HGLGPS fluorescence light source provides bright, even illumination and features a long life, 2,000-hour mercury burner (average lifetime). The unit does not require maintenance and centering adjustment. A liquid light guide helps to prevent heat transfer to samples, thus allowing prolonged observations to be performed without concern.
  • Measuring Excitation Light Intensity for High Reproducibility in Imaging Adapter for Excitation Irradiance Meter/IX3-EXMAD*: Evident currently offers an adapter for a power meter that can immediately measure the excitation light intensity per unit surface area of the sample, as well as providing irradiance display software.
    It displays the measurement results on a monitor and records the data, thus eliminating the need for arduous calculations. This facilitates checking of the excitation light intensity prior to starting an experiment, improving the reliability of experiments. It is possible to easily share data.

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High-Resolution, Automated Inverted Microscope - IX83

IX83 Two-deck System
IX83 One-deck System
Reliable, Clear and Bright High-Resolution Images
HeLa cell expressing mCherry-actin
3D reconstruction images of a live sphere made of NMuMG-Fucci2 cells
Special Objective Available for iPS/ES and Floating Cell Observation
Special Objective Available for iPS/ES and Floating Cell Observation
With Fly-Eye Lens System
Without Fly-Eye Lens System
Image Captured with a Conventional FL Mirror Unit
Images Captured with New FL Mirror Units
Images Captured with New FL Mirror Units
IX83 Touch Panel Controller
ZDC One-Shot Function Quickly Detects Focus, Even during High Magnification Observation
Intuitive Microscope and XY Stage Controllers
Evident cellSens Imaging Software
Reliable Tracking at High Magnification
Easily Achieve Koehler Illumination
Catch Tray Minimizes Optical System Contamination
Easily Capture Time-Lapse Images
Easily Capture Time-Lapse Images
Easily Capture Time-Lapse Images
IX3-ZDC Z drift compensation
Z Drift Compensation System
IX3-HOW-2, IX3-HOS and IX3-HO35D
Efficient Multi-Image Acquisition with cellSens Software
Efficient Multi-Image Acquisition with cellSens Software
Motorized DIC Slider for IX83
Interchangeable Modules Provide Flexible Imaging Options
Interchangeable Modules Provide Flexible Imaging Options
Motorized Fast Filter Wheels and Shutters
Application-Specific Fluorescence Illuminators
Long Life 130W Mercury Lamp (U-HGLGPS)
Imaging Adapter for Excitation Irradiance Meter

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