The Future of Fluorescence
Duetta is an exciting new bench-top instrument from Horiba Scientific.
Two-in-One Fluorescence and Absorbance Measurements
This innovative spectrometer allows the measurement of both fluorescence and absorbance, and can also be used either just as fluorometer, or as UV-Vis-NIR spectrometer for absorbance measurement
Duetta has an automatic real time Inner Filter Effect (IFE) correction for both Primary and Secondary IFE, which is especially important for obtaining a true molecular fingerprint with simultaneous measurement.
A new fingerprinting technique, called A-TEEM, combines Absorbance-Transmission and EEM (excitation−emission matrix).
Fast and Accurate
Duetta is equipped with State-of-the-art Charge Coupled Device (CCD Detector) instead of traditionally used photomultiplier tubes (PMT) for fluorescence detection.
This technology allows detection of entire fluorescence spectrum in just a millisecond. A full 3D fluorescence EEM can be acquired in less than a second.
CCD detector also extends NIR wavelength detection to 1100 nm.
Entire wavelength range of Duetta is from 250 nm to 1100 nm for UV-Vis-NIR Fluorescence detection.
The highest level of florescence sensitivity allows accurate measurement even for low concentration samples.
Touch-Screen Software EzSpecTM
Another innovation from Horiba Scientific embedded in Duetta is touch-screen software interface with selection of Apps for routine analysis.
Duetta Highlights
- Simultaneous measurement of fluorescence and absorbance
- Real time Inner Filter Effect correction for both Primary and Secondary IFE
- Fast detection with Charge Coupled Device: entire fluorescence spectrum in just a millisecond
- Detection range from 250 nm to 1100 nm
- A-TEEM fingerprinting combines Absorbance-Transmission and EEM
- EzSpecTM touch-screen software interface
Specifications
| |
Duetta |
Duetta-Bio |
| Acquisition Modes |
Simultaneous Absorbance and Fluorescence Spectrometer |
| Fluorescence Sensitivity |
SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits |
| Spectral Acquisition Rate |
510,000 nm/min |
| EEM Acquisition Rate |
As fast as 1 second (sample and wavelength dependent) |
| A-TEEM Acquisition Rate |
As fast as 30 seconds (sample and wavelength dependent) |
| Fluorescence Detector |
CCD / Spectrograph |
| Fluorescence Detector Range |
250-1,100 nm |
| Fluorescence Bandwidth |
1, 2, 3, 5,10, 20 nm (excitation and emission) |
| Absorbance Detector |
Silicon Photodiode |
| Absorbance Detector Range |
250-1,000 nm |
| Absorbance Bandwidth |
2, 3, 5, 10 nm |
| Light Source |
75 W Xenon arc lamp. Dedicated cartridge for snap-in replacement |
| Excitation/Absorbance Wavelength Range |
250-1,000 nm |
| Absorbance Accuracy |
+/- 0.02 A |
| Wavelength Accuracy |
+/- 1 nm |
| Absorbance Range |
0-2 A |
| Computer Requirements |
Windows® 10/8/7 Laptop or PC with 1 USB |
| Software |
EzSpec |
| Dimensions (W x D x H) |
17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm |
| Weight |
45 pounds; 20.4 kg |
| Polarizers (Time of Purchase) |
Not Available |
Yes (280 to 750 nm) |
Duetta - Horiba