Fluorescence and Absorbance Spectrometer - Duetta™

Duetta is an exciting new bench-top instrument from Horiba Scientific.

Two-in-One Fluorescence and Absorbance Measurements

This innovative spectrometer allows the measurement of both fluorescence and absorbance, and can also be used either just as fluorometer, or as UV-Vis-NIR spectrometer for absorbance measurement

Duetta has an automatic real time Inner Filter Effect (IFE) correction for both Primary and Secondary IFE, which is especially important for obtaining a true molecular fingerprint with simultaneous measurement.

A new fingerprinting technique, called A-TEEM, combines Absorbance-Transmission and EEM (excitation−emission matrix).

Fast and Accurate

Duetta is equipped with State-of-the-art Charge Coupled Device (CCD Detector) instead of traditionally used photomultiplier tubes (PMT) for fluorescence detection.

This technology allows detection of entire fluorescence spectrum in just a millisecond. A full 3D fluorescence EEM can be acquired in less than a second.

CCD detector also extends NIR wavelength detection to 1100 nm.

Entire wavelength range of Duetta is from 250 nm to 1100 nm for UV-Vis-NIR Fluorescence detection.

The highest level of florescence sensitivity allows accurate measurement even for low concentration samples.

Touch-Screen Software EzSpecTM

Another innovation from Horiba Scientific embedded in Duetta is touch-screen software interface with selection of Apps for routine analysis.

Duetta Highlights

  • Simultaneous measurement of fluorescence and absorbance
  • Real time Inner Filter Effect correction for both Primary and Secondary IFE
  • Fast detection with Charge Coupled Device: entire fluorescence spectrum in just a millisecond
  • Detection range from 250 nm to 1100 nm
  • A-TEEM fingerprinting combines Absorbance-Transmission and EEM
  • EzSpecTM touch-screen software interface


  Duetta Duetta-Bio
Acquisition Modes Simultaneous Absorbance and Fluorescence Spectrometer
Fluorescence Sensitivity SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits
Spectral Acquisition Rate 510,000 nm/min
EEM Acquisition Rate As fast as 1 second (sample and wavelength dependent)
A-TEEM Acquisition Rate As fast as 30 seconds (sample and wavelength dependent)
Fluorescence Detector CCD / Spectrograph
Fluorescence Detector Range 250-1,100 nm
Fluorescence Bandwidth 1, 2, 3, 5,10, 20 nm (excitation and emission)
Absorbance Detector Silicon Photodiode
Absorbance Detector Range 250-1,000 nm
Absorbance Bandwidth 2, 3, 5, 10 nm
Light Source 75 W Xenon arc lamp. Dedicated cartridge for snap-in replacement
Excitation/Absorbance Wavelength Range 250-1,000 nm
Absorbance Accuracy +/- 0.02 A
Wavelength Accuracy +/- 1 nm
Absorbance Range 0-2 A
Computer Requirements Windows® 10/8/7 Laptop or PC with 1 USB
Software EzSpec
Dimensions (W x D x H) 17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm
Weight 45 pounds; 20.4 kg
Polarizers (Time of Purchase) Not Available Yes (280 to 750 nm)


Duetta - Horiba

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