Museum quality mounting media, permanent clarity, strictly controlled optical specifications.
Histomount is the classic choice in synthetic mounting media. It provides a permanent seal so that ships can be stored or shipped with confidence.
Histomount has a neutral pH, is UV stabilized, and retains its brilliance and clarity for several years. Its refractive index is matched to that of glass cover slips and slide, reducing chromatic aberration with any light source. It is effective with most clearing agents when used as a liquid cover slip or as a permanent mounting medium for glass cover slips. Each 100 ml bottle includes a dip stick to ensure an optimal amount of Histomount.
Shur/Mounttm Toluene-Based Liquid Mounting Medium
Used for mounting and preserving microscope slide samples
Mount-Quick Mounting Medium in a Tube
It provides complete control over the dispensed drops and its density does not change. Mount-Quick dries rapidly with no bubbles and is highly transparent. It is soluble in xylene and is a unique tissue transfer medium.
- Density: 40% solids
- Specific gravity: 0.89 SP
- Viscosity: 33 cP (25 °C)
||Mount-Quick, Mounting Medium in a Tube, 30 ccm
Cargill Meltmounttm for Microscopy and Optical Coupling
These mounting media are specially designed optical-grade thermoplastics for use in microscope slide mounting and in other optical coupling applications.
- Instant - they are thermal mounts and require "no oven time"
- 100% usable - contain no solvents
- Less expensive per slide
- Thermally reversible for particle retrieval or re-orientation
- Soluble in toluene if needed for special techniques or clean-up
- Fluid at 65 °C - a temperature chosen because it makes a permanent mount and protects the majority of specimens from thermal changes.
- Direct replacements for "older style" media
Notes of use:
Meltmount is a thermal plastic, which means its viscosity decreases as the temperature increases. It has no sharp melting point. It is capable of “cold flow,” which means the Meltmount, sample, slide, and the cover slip can all move independently of each other given a mix of time, temperature, and lateral pressure or gravity.
Storage of prepared slide:
- Flat, cover slip on top
- In the dark,
- Away from dust and fumes
- At 60 to 85 °F. Storing or transporting the prepared slide above 85 °F may reverse the mounting as the media is thermally reversible.
Cargille MeltmountTM is available in a convenient form called Quick-Stick. It can be used to quickly make permanent microscope slide mounts. Quick-StickTM can be applied to a slide using a hotplate. The permanent prepared slide is ready once the sample and cover glass are positioned and the slide is cooled. The mounting can be reversed by reheating.
With a refractive index of 1.539 (at 25 °C) and an Abbe V dispersion of 45, this is similar to Canada Balsam, making it ideal for mounting many biological samples but without the long drying time of Canada Balsam.
MeltmountTM for Chrysotile Asbestos
Its refractive index (at 25 °C) of 1.550 and dispersion characteristics make it suitable for mounting chrysotile asbestos.
With a refractive index of 1.582 (at 25 °C) and an Abbe V dispersion of 33, its optical clarity makes it suitable for low visible absorption applications.
A refractive index of 1.605 (at 25 °C) and an Abbe V dispersion of 30 make it suitable for mounting asbestiform Tremolite, Anthophyllite, and Actinolite.
Its refractive index (at 25 °C) of 1.662 and an Abbe V dispersion of 26 make it similar to Aroclor 5442, but is PCB-free.
A refractive index of 1.680 (at 25 °C) and an Abbe V dispersion of 25 make it suitable for mounting amolite and crosidolite asbestos.
Its refractive index (at 25 °C) of 1.704, similar to naphrax, and an Abbe V dispersion of 24.
Mounting Media/Balsam Bottle
It has a glass applicator block and glass cap, making it suitable for applying mounting media. The cap is ground to the shoulder of the bottle to make a seal. Made of “810” soda lime glass. Capacity: 100 ml. Diameter: 17 mm (.67"). Height: 100 mm (3.94"). (supplied without media).
Aqueous Mounting Media
Mount-Quick Aqueous Mounting Medium
Mount-Quick Aqueous, available in a 30 ml bottle, is a water-based mounting media that can be used with lipid sections, immunohistochemical stained sections, and AEC method stains.
- Refractive index: 1.41
- Specific gravity: 0.88
- Viscosity: 33 cps at 25 °C
- pH: 7.0
Shur/MountTM Liquid Coverglass and Mounting Medium
SHUR/MountTM Aqua-Poly/Mount is designed to place coverslips on slides directly from water. Since it is water based and non-fluorescing, it can be used for immune-fluorescent techniques as it enhances and restains fluorescent stains.
Fluoromount-GTM Mounting Media
It is a water-soluble non-fluorescing mounting media for cover slipping slides after a staining procedure with an aqueous final step. This mounting media has a semi-permanent seal for long-term storage and may be used with cell preparations and tissue sections.
Citifluor Antifadent Solutions
Citifluor is a range of antifadent mounting media that greatly reduces fluorescence fading from FITC, rhodamine, Hoechst, Alexa, Green Fluorescent Protein, cyanine dyes, Texas Red, and phycoerythins. These mountants are available in either glycerol-based or glycerol-free solutions and can be hardening (permanent) or non-hardening.
Fluorescent labels and markers are used widely now and immuno-fluorescence, a method that uses antibodies tagged with labels such as fluorescein, is a well-established technique.
However, a general problem during examining these materials under the microscope is the light used for fluorescence stimulation also degrades the label, causing the fluorescence to fade. This also affects seeing materials that have a low labeling level. This photofading of labels can be slowed using Citifluor mountants.
Antifadent (AF) Solutions
Although antifadent solutions AF-1, AF2, and AF-3 were developed for use with FITC polyclonal antibodies, they are being widely used as they can preserve the signal for other fluorochromes such as rhodamine and DAPI. AF-4 contains the antifadent propyl gallate, which is particularly suited for use with DAPI and Alexa dye-stained materials, as well as FITC-labeled materials.
AF-1 Glycerol-phosphate buffered solution containing antifadent for use with labeled tissue and dead cells. Available in two sizes.
Citifluor Aqueous Mounting Media
|Gycerol based solutions
||AF-1, Glycerol-PBS, with antifadent designed for antibodies labeled with FITC, also prevents photobleaching of DAPI, rhodamines, Hoechst, Alexa, cyanine dues, Texas Red, and GFP, 25 ml and 100 ml
||AF-2, Glycerol with an antifadent optically transparent from 300 to 750nm, stops photobleaching of DAPI, rhodamines, Hoechst, Alexa, cyanine dues, Texas Red, and GFP, 25 ml and 100 ml
|Glycerol solution, additives.
||AF-4, Glycerol with an antifade reagent n-propyl gallate, reduces photobleaching of rhodamine and FITC, optically transparent from 400 to 750nm, 25 ml
||AF-200 Glycerol solution containing a non-basic antifadent, high viscosity and designed for use with specimens labeled with DAPI, CY and Hoechst dye, 10 ml
||AF-300 Glycerol solution of an amine-based antifadent, high viscosity and designed for use with a wide range of fluorochromes, 10 m
|Glycerol-based solutions with high refractive index (~1.52)
||CFM1 Glycerol-PBS solution used for transmission and epifluorescence microscopy, 10 ml
||CFM1/AF Glycerol-PBS solution + antifadent, 10 ml
||CFM2 Glycerol-tris amine buffered solution, pH 8.5, 10 ml
||CFM3 Glycerol-based solution that contains an antifadent that isn’t an amine, 10 ml
|Glycerol free solutions
||AF-3 PBS solution containing an amine-based antifadent for live cell imaging, 25 ml and 100 ml
||AF-100 PBS solution with amine-based antifadent, 5 ml
NOTE: can be used with 19497
||UVM1 methacrylate-based solution on exposure to light hardens to clear film. Totally water miscible (ratio of UVM1 to AF100 10:1), 10 ml
||UVM2 methacrylate based solution containing an anti-bleaching agent, which on exposure to light hardens to give clear film. It is totally water miscible, 10 ml
||Citifluor Solid Mountant Kit – 25 ml PVA, PBS solution with anti-fadent, 30 ml
Components are mixed, applied to specimen and allowed to dry to a clear film.