The TriVersa NanoMate incorporating ESI Chip® Technology Liquid Extraction Surface Analysis (LESA®) is the most recent offering from Advion in chip-based electrospray ionization technology. It integrates the advantages of mass spectrometry, liquid chromatography, fraction collection, chip-based infusion, and direct surface analysis into a single integrated ion source platform. When compared to LC/MS alone, it enables researchers to acquire more information from complex samples.
Being the only automated ion source platform, the TriVersa NanoMate enables high-throughput infusion experiments for large or small analytes in applications as wide-ranging as shotgun lipidomics and non-covalent interaction studies (NCI) to protein characterization. The TriVersa NanoMate has been designed to function as a nano-ESI ion source for LC/MS applications. In the case of additional fraction collection, it is possible to use excess LC eluate to form a physical storage of the LC/MS experiment, where the eluate enables re-analysis of fractions of interest as recognized by the online LC/MS run.
By means of miniaturization, the ESI Chip unleashes the power of mass spectrometry. This microfluidic chip includes 400 nano-electrospray nozzles that are etched in a silicon wafer. The fabrication technique is highly reproducible, thus guaranteeing the integrity and equal performance of each nozzle. The electrical field generated by the nano-electrospray nozzle leads to a highly efficient ionization and stable, long-lasting sprays from microliters of sample volume.
LESA and LESA Plus LC
Liquid Extraction Surface Analysis (LESA) was devised in partnership with Dr Gary van Berkel from Oak Ridge National Laboratories. Its specialized surface extraction through a liquid droplet held in suspension (liquid junction) is an innovative technique for spatially resolved analysis of technical and biological surfaces of interest.