Capel-205 , a new generation capillary electrophoresis (CE) system from Lumex Instruments, is able to detect all types of components in a wide range of application areas. It can be the perfect choice not just for low-scale analyses and R&D but also for customers handling a number of samples every day.
Principle of Operation The basis of high-performance capillary electrophoresis is performed through the differential migration of components of aqueous samples within a narrow fused silica capillary to which an electric field is applied. Quantitative detection of the separated solutes is performed at the capillary outlet using a high-sensitive optical system on the basis of direct or indirect UV absorbance.
Features and Benefits Comprehensive instrumental options
Testing of numerous samples per month enabled by extended autosampler capacity of up to 59 positions
Voltage up to ±30 kV is provided by the new HV block, thus reducing analysis time and increasing separation efficiency
Integrated unit for the removal of tube caps opens samples exactly prior to the analysis, thereby eliminating evaporation and cross-contamination
Peak identification enabled by spectra scanning
Migration time reproducibility is enhanced by the redesigned capillary cassette with increased length of the capillary’s thermostated part
Reverse injection of samples under vacuum: ultra-short analysis time (<1 minute) and sample stacking to minimize detection limit
Unique design of the capillary cassettes
Possibility to change capillary easily within a few minutes
The capillary’s enlarged thermostatic part improves migration time reproducibility
Detection limit reduced by optimized optical scheme
Precise liquid temperature control (±0.1 °C)
Optimized separation efficiency and extended range of applied buffers
Robust software package Elforun
Real-time electropherogram visualization
Combination of instrument control, data acquisition, and data processing
Computation of electrophoresis system parameters
Processing of electropherogram data with overlay mode and established mathematical operations
Possibility of all types of complex runs including those with pre-programming of changes in analysis conditions
Extensive data processing algorithm customization
Personalized report, data export to other programs, data exchanges with databases, worksheets, and word processors
GLP and GALP compliance
Streaming potential control technique
Enhancing migration time repeatability and accuracy of analysis
Potential to carry out overnight analyses
Analytical Characteristics Detectors
Capel-205 is equipped with a spectrophotometric detector with a wavelength range of 190–400 nm
Light source
Tubes
Standard 1.5-mL centrifuge type, minimal sample volume of 100 µL
Injection modes
Samples injected by pressure, vacuum, or electrokinetically
Electrokinetic injection performed at (±) 1–30 kV with respect to the time for voltage ramping
Controlled pressure profile of injection with pressure values from −100 mbar to +100 mbar
Programmable injection time/pressure/voltage
Analysis
Voltage from −30 kV to +30 kV in steps of 1 kV and an electronic polarity switch
Current from 0 to 300 µA
Equipment and Options
Capillary electrophoresis system Capel-205
Elforun software package
Kits for analysis (by request); most of the CE-kits from any manufacturer match with Capel-205
Additional capillary cassettes
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