Capel-205, a new generation capillary electrophoresis (CE) system from Lumex Instruments, is able to detect all types of components in a wide range of application areas. It can be the perfect choice not just for low-scale analyses and R&D but also for customers handling a number of samples every day.
Principle of Operation
The basis of high-performance capillary electrophoresis is performed through the differential migration of components of aqueous samples within a narrow fused silica capillary to which an electric field is applied. Quantitative detection of the separated solutes is performed at the capillary outlet using a high-sensitive optical system on the basis of direct or indirect UV absorbance.
Features and Benefits
Comprehensive instrumental options
- Testing of numerous samples per month enabled by extended autosampler capacity of up to 59 positions
- Voltage up to ±30 kV is provided by the new HV block, thus reducing analysis time and increasing separation efficiency
- Integrated unit for the removal of tube caps opens samples exactly prior to the analysis, thereby eliminating evaporation and cross-contamination
- Peak identification enabled by spectra scanning
- Migration time reproducibility is enhanced by the redesigned capillary cassette with increased length of the capillary’s thermostated part
- Reverse injection of samples under vacuum: ultra-short analysis time (<1 minute) and sample stacking to minimize detection limit
Unique design of the capillary cassettes
- Possibility to change capillary easily within a few minutes
- The capillary’s enlarged thermostatic part improves migration time reproducibility
- Detection limit reduced by optimized optical scheme
Precise liquid temperature control (±0.1 °C)
- Optimized separation efficiency and extended range of applied buffers
Robust software package Elforun
- Real-time electropherogram visualization
- Combination of instrument control, data acquisition, and data processing
- Computation of electrophoresis system parameters
- Processing of electropherogram data with overlay mode and established mathematical operations
- Possibility of all types of complex runs including those with pre-programming of changes in analysis conditions
- Extensive data processing algorithm customization
- Personalized report, data export to other programs, data exchanges with databases, worksheets, and word processors
- GLP and GALP compliance
Streaming potential control technique
- Enhancing migration time repeatability and accuracy of analysis
- Potential to carry out overnight analyses
Analytical Characteristics
Detectors
- Capel-205 is equipped with a spectrophotometric detector with a wavelength range of 190–400 nm
Light source
Tubes
- Standard 1.5-mL centrifuge type, minimal sample volume of 100 µL
Injection modes
- Samples injected by pressure, vacuum, or electrokinetically
- Electrokinetic injection performed at (±) 1–30 kV with respect to the time for voltage ramping
- Controlled pressure profile of injection with pressure values from −100 mbar to +100 mbar
- Programmable injection time/pressure/voltage
Analysis
- Voltage from −30 kV to +30 kV in steps of 1 kV and an electronic polarity switch
- Current from 0 to 300 µA
Equipment and Options
- Capillary electrophoresis system Capel-205
- Elforun software package
- Kits for analysis (by request); most of the CE-kits from any manufacturer match with Capel-205
- Additional capillary cassettes