FLUOstar Omega Filter-Based Multi-Mode Microplate Reader

The FLUOstar® Omega is an ideal plate reader for all life science applications. It is a multi-mode microplate reader, with six detection modes. For absorbance, it uses an ultra-fast UV/vis spectrometer or filters, and highly sensitive filters for all other detection modes.

Product Details

The six detection modes used in the versatile FLUOstar® Omega multi-mode plate readerare: fluorescence intensity (including FRET), luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®.

Features

  • High-speed absorbance measurements
  • Measurements in up to 384-well plates
  • Top and bottom reading
  • Incubation up to 45 or 65 °C
  • User-defined shaking
  • Well scanning with up to 900 data points per well
  • High-precision injectors with simultaneous reagent injection and detection
  • Low-volume measurements

Technical Specifications

Detection modes

Fluorescence intensity - incl. FRET

AlphaScreen®/AlphaLISA®

Luminescence (flash and glow) - incl. BRET

Time-resolved fluorescence

TR-FRET

UV/Vis absorbance spectra

Measurement modes

Top and bottom reading

Endpoint and kinetic

Sequential multi excitation

Sequential multi emission

Spectral scanning (absorbance)

Ratiometric measurements

Well scanning

Microplate formats

6 to 384-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate carrier

Robot compatible

Light sources

High energy xenon flash lamp

Detectors

Low noise photomultiplier tube
CCD spectrometer

Wavelength selection

Optical filters: ex and em wheels for 8 filters each
UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 s/well

Optical filters

Excitation and emission filter wheels for 8 filters each

Optical path guides

Top: liquid-filled light guides
Bottom: fiber optics

Spectral range

Filters: 240–740 nm or 240–900 nm for FI, FP, TRF
240–740 nm for LUM

Spectrometer: 220–1000 nm for ABS

Sensitivity

FI

< 0.2 fmol/well FITC

TRF

< 30 amol/well europium

High-End TRF for Omega

< 3 amol/well europium

LUM

< 20 amol/well ATP

AlphaScreen®

< 5 pM (< 100 amol/well P-Tyr-100, 384 sv, 20 μL)

ABS
with

spectrometer

Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1, 2, 5 and 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

 

Read times

Flying mode (1 flash), 9 s (96), 16 s (384)

Reagent injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge gas vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

 

Optional Accessories

LVis plate

Sample capacity: 16 separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.

Quality control internal standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates.

Stacker

Magazines for up to 50 plates - continuous loading feature

THERMOstar

Microplate incubator and shaker

Optical filters

Excitation and emission filters

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.

 

LOD = 3x SD (20 blanks)/slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384 sv = 384-well small volume microplates
384 g = 384-well glass bottom microplates

 

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