WITec alpha300 Ri - Inverted Confocal Raman Imaging Microscope

The alpha300 Ri inverted confocal Raman imaging microscope from WITec makes 3D chemical characterization possible from a new angle.

The inverted beam path of the alpha300 Ri microscope preserves all the functionality of WITec’s alpha300 R Raman microscope series while introducing even greater flexibility in sample access and handling. The ability to survey and analyze samples from below is a considerable advantage when investigating aqueous solutions and bulky samples. Researchers in the fields of pharmaceutics, geoscience, life sciences and many more will benefit from the versatility and user-friendliness offered by the alpha300 Ri.

Key Features

  • An inverted beam path enables liquid samples to be placed on the fixed plane of the stage for both rapid and repeatable measurements
  • The motorized sample stage allows for the integration of environmental enclosures and other accessories
  • Raman microscopy is non-destructive and doesn't require staining or other specialiized sample preparation
  • Large samples that would be difficult to analyze under a traditional microscope objective turret can be accommodated by the alpha300 Ri’s sample stage
  • Features all the exclusive and proven imaging and spectroscopy capabilities of the WITec alpha300 R range
  • Can be used with other microscopy methods such as fluorescence, phase-contrast, and differential interference contrast (DIC)


Correlative Raman - fluorescence microscopy image of eukaryotic cells. Nuclei were stained with DAPI (blue). Endoplasmic reticulum (red) and nucleoli (green) were identified by their Raman signals.

Correlative Raman - fluorescence microscopy image of eukaryotic cells. Nuclei were stained with DAPI (blue). Endoplasmic reticulum (red) and nucleoli (green) were identified by their Raman signals. Image Credit: WITec GmbH

3D Raman measurement of banana pulp. Starch grains (green) and cell wall components (red) are clearly visible

3D Raman measurement of banana pulp. Starch grains (green) and cell wall components (red) are clearly visible. Image Credit: WITec GmbH

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