Determination of critical product parameters for proteins from research to production

During this webinar the diversity of various light scattering techniques for characterizing a wide range of protein samples and their applicability in pharmaceutical research will be shared.

We will start by highlighting the potential of dynamic light scattering (DLS) as a tool for rapid and straightforward aggregate detection, as well as its value in executing extensive formulation studies and predicting stability.

This will be followed by applications of multi-angle light scattering (SEC-MALS and FFF-MALS), showcasing its ability to provide absolute molecular weight, oligomerization, and conjugate analyses.

We will conclude with an introduction to real-time MALS, offering real-time insights and enabling direct process influence on attributes such as molar mass, size, and particle concentration.

Key Learning Objectives:

  • Learn about the many ways light scattering detection can be used to characterize proteins, peptides, and protein conjugates.
  • Introduction to Dynamic Light Scattering (DLS) for aggregate detection, formulation screening and stability prediction.
  • Multi-Angle Light Scattering coupled to Size Exclusion Chromatography (SEC-MALS) for determination of the molar mass distribution of protein samples, to gain insights into the oligomeric state, and to characterize conjugated samples.
  • Explanation of of Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering as an orthogonal method to SEC-MALS.
  • Introduction to Real-Time MALS for process development and manufacturing.

About the webinar speakers: 

David Golonka is an Application Scientist at the Analytical Laboratory of Wyatt Technology Europe GmbH, the leading provider of light scattering instruments for the solution-based characterization of macromolecules and nanoparticles.

He studied chemistry and biochemistry and later received his Ph.D. degree in Protein Biochemistry from the University of Bayreuth (Germany) where he did research on photoreceptor proteins.

His role at Wyatt Technology Europe involves application consulting, method development and contract research in close cooperation with outstanding customers from the pharmaceutical and chemical industries as well as renowned research institutes and universities. 

Dr. Felix Deluweit carried out his doctorate working on propagation and characterization of prion strains at the Helmholtz-Centre for Infection Research.

He joined Wyatt Technology in 2013 as a scientist in Wyatt’s application lab. Currently, he is Account Manager for North & East Germany and East-Europe and has strong expertise in SEC-MALS, FFF-MALS, DLS and Real-time light scattering.

Who Should Attend this Webinar?

Scientists working in the field of protein characterization, protein formulation and process development.

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