The DynaPro Zetastar is an instrument that combines measurements of dynamic and static light scattering (DLS/SLS) or dynamic and electrophoretic light scattering (DLS/ELS) simultaneously.

Microcuvette measurements can be carried out manually or automatically using a flow cell and an autosampler. This can swiftly evaluate parameters for characterization and stability investigations, including size, polydispersity, zeta potential, charge, particle concentration, molar mass, or turbidity.

Key Features

  • DYNAMICS Touch on-board application for self-directed, walk-up operation
  • With as little as 2 µL of solution, users can assess size and turbidity, or zeta potential with 65 µL of solution
  • Adaptive collection for zeta potential measurements that are quicker and easier
  • In buffers with conductivity up to two times physiological saline, measure the zeta potential
  • A variety of high-quality disposable cells, cuvettes, and quartz cells for zeta potential and size measurements


Source: Waters | Wyatt Technology

test test
Pressure Range Flow cell pressurizable to 400 psi for bubble suppression in conductive solvents
Optical System Multiple optical channels: in-cuvette DLS & SLS, in-cuvette or flow cell DLS & ELS, fiber connection to Wyatt MALS detector for online DLS.
ELS optical system: fiber-coupled interferometric Doppler electrophoretic light scattering (FIDELIS), determines electrophoretic mobility and zeta potential from frequency shift of scattered light
Refractive Index Range N/A
Noise Level N/A
Plate Formats N/A
Measurement Mode(s) ELS: electrophoretic mobility, zeta potential, electrical charge
DLS: size, size distributions, polydispersity
SLS: particle concentration, molar mass, turbidity
Applications Analysis of proteins, gene vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, nano-emulsions, lipid and metallic nanoparticles, and more.
Linearity SLS detector is linear over 6 decades
Principle Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS)
Sensitivity DLS: 0.1 mg/mL of lyozyme
ELS: 1 mg/mL BSA
SLS: 0.1 mg/mL lysozyme
Measuring Range Size: 0.2 nm to 1000 nm (hydrodynamic radius)
Zeta potential: No practical limit
Molecular weight: 300 Da to 1 MDa
Wavelength(s) 785 nm
Type Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS)
Stability N/A
Measuring Time 15 seconds typical
Radius of Gyration Range N/A
Detection System DLS: Avalanche photodiode at 90º or 163.5º scattering angle
SLS: photodiode at 90º scattering angle
ELS Detection: photodiode at 10º scattering angle for FIDELIS (fiber-interferometric Doppler electrophoretic light scattering)
Data Acquisition Rate N/A
Sample Volume Quartz Cuvette: 2 µL and 45 µL
PEEK Flow Cell: 350 µL
Dip Cell: 65 µL
Disposable DLS Cuvette: 4 µL
Disposable Zeta Cell: 400 µL
Max. Flow Rate N/A
Dimensions (WxDxH) 36 x 57 x 20 cm
Shear Rate Range N/A
Max. Pressure N/A
Detector(s) N/A
Temperature Range -10 °C to +120 °C
Interface DYNAMICS TOUCH onboard app or DYNAMICS Software on PC
Sample Type Proteins, polymers, and nanoparticles including viruses, liposomes, gene vectors, nanoemulsions, etc.
Cell Volume N/A
Control Software control of detector and autosampler system to automate a series of unattended measurements
Particle Size 0.2 to 1000 nm
Light Source 785 nm laser, programmable 10% to 100%
Dynamic Range N/A

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