The DynaPro Zetastar is an instrument that combines measurements of dynamic and static light scattering (DLS/SLS) or dynamic and electrophoretic light scattering (DLS/ELS) simultaneously.
Microcuvette measurements can be carried out manually or automatically using a flow cell and an autosampler. This can swiftly evaluate parameters for characterization and stability investigations, including size, polydispersity, zeta potential, charge, particle concentration, molar mass, or turbidity.
Key Features
- DYNAMICS Touch on-board application for self-directed, walk-up operation
- With as little as 2 µL of solution, users can assess size and turbidity, or zeta potential with 65 µL of solution
- Adaptive collection for zeta potential measurements that are quicker and easier
- In buffers with conductivity up to two times physiological saline, measure the zeta potential
- A variety of high-quality disposable cells, cuvettes, and quartz cells for zeta potential and size measurements
Specifications
Source: Waters | Wyatt Technology
| test |
test |
| Pressure Range |
Flow cell pressurizable to 400 psi for bubble suppression in conductive solvents |
| Optical System |
Multiple optical channels: in-cuvette DLS & SLS, in-cuvette or flow cell DLS & ELS, fiber connection to Wyatt MALS detector for online DLS.
ELS optical system: fiber-coupled interferometric Doppler electrophoretic light scattering (FIDELIS), determines electrophoretic mobility and zeta potential from frequency shift of scattered light |
| Refractive Index Range |
N/A |
| Noise Level |
N/A |
| Plate Formats |
N/A |
| Measurement Mode(s) |
ELS: electrophoretic mobility, zeta potential, electrical charge
DLS: size, size distributions, polydispersity
SLS: particle concentration, molar mass, turbidity |
| Applications |
Analysis of proteins, gene vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, nano-emulsions, lipid and metallic nanoparticles, and more. |
| Linearity |
SLS detector is linear over 6 decades |
| Principle |
Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS) |
| Sensitivity |
DLS: 0.1 mg/mL of lyozyme
ELS: 1 mg/mL BSA
SLS: 0.1 mg/mL lysozyme |
| Measuring Range |
Size: 0.2 nm to 1000 nm (hydrodynamic radius)
Zeta potential: No practical limit
Molecular weight: 300 Da to 1 MDa |
| Wavelength(s) |
785 nm |
| Type |
Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS) |
| Stability |
N/A |
| Measuring Time |
15 seconds typical |
| Radius of Gyration Range |
N/A |
| Detection System |
DLS: Avalanche photodiode at 90º or 163.5º scattering angle
SLS: photodiode at 90º scattering angle
ELS Detection: photodiode at 10º scattering angle for FIDELIS (fiber-interferometric Doppler electrophoretic light scattering) |
| Data Acquisition Rate |
N/A |
| Sample Volume |
Quartz Cuvette: 2 µL and 45 µL
PEEK Flow Cell: 350 µL
Dip Cell: 65 µL
Disposable DLS Cuvette: 4 µL
Disposable Zeta Cell: 400 µL |
| Max. Flow Rate |
N/A |
| Dimensions (WxDxH) |
36 x 57 x 20 cm |
| Shear Rate Range |
N/A |
| Max. Pressure |
N/A |
| Detector(s) |
N/A |
| Temperature Range |
-10 °C to +120 °C |
| Interface |
DYNAMICS TOUCH onboard app or DYNAMICS Software on PC |
| Sample Type |
Proteins, polymers, and nanoparticles including viruses, liposomes, gene vectors, nanoemulsions, etc. |
| Cell Volume |
N/A |
| Control |
Software control of detector and autosampler system to automate a series of unattended measurements |
| Particle Size |
0.2 to 1000 nm |
| Light Source |
785 nm laser, programmable 10% to 100% |
| Dynamic Range |
N/A |