Automatic Calibration of Analytes Using a Combination of Inline Dilution and Subsequent Inline Ultrafiltration

Metrohm inline sample preparation (MISP) is a versatile collection of cost and time-effective techniques that reduce manual sample preparation. This article discusses a combination of inline dilution and subsequent inline ultrafiltration which allows automatic dilution and subsequent filtration of samples before injection.

MagIC Net allows intelligent dilution to be run by checking results and the sample to be re-diluted if the result is not within the calibration range. In addition, the stock standard solution is automatically diluted for multipoint calibration. As a result, each analyte can be automatically calibrated effectively.

Download the Article

Sample

Mixed standard stock solution (1000 mg/L of each anion)

Sample preparation

Metrohm inline dilution technique (MIDT) followed by Metrohm inline ultrafiltration.

Columns

. .
Metrosep A Supp 5 - 100/4.0 6.1006.510
Metrosep A Supp 5 Guard/4.0 6.1006.500

Solutions

Eluent 3.2 mmol/L sodium carbonate
1.0 mmol/L sodium hydrogen carbonate
Suppressor regenerant 100 mmol/L sulfuric acid
Rinsing solution STREAM

Analysis

Conductivity detection following the sequential suppression.

Instrumentation

930 Compact IC Flex Oven/SeS/PP/Deg 2.930.2560
IC Conductivity Detector 2.850.9010
858 Professional Sample Processor 2.858.0020
800 Dosino 2.800.0010
741 Magnetic Stirrer 2.741.0010
IC equipment: Inline Dilution 6.5330.120
IC equipment: Inline Ultrafiltration 6.5330.130
MSM Rotor A 6.2832.000
Adapter sleeve for Suppressor Vario 6.2842.020

Parameters

. .
Flow rate 0.7 mL/minutes
Injection volume 20 μL
Pmax 15 MPa
Recording time 11.5 minutes
Column temperature 45 °C
Final dilution volume 7 mL

Calibration

.
Stock standard solution 50.0 mg/L of chloride, nitrate, and sulfate each
Dilution factor Calibration level
Calibration level Dilution factor Concentration
Level 1 1 50.0 mg/L
Level 2 2 25.0 mg/L
Level 5 5 10.0 mg/L
Level 10 10 5.00 mg/L
Level 20 20 2.50 mg/L
Level 50 50 1.00 mg/L

Setup Scheme

The installation is carried out in accordance with the AB 376 ProfIC Vario 6 Anion.

Results

Anion Calibration
RSD [%]
Calibration
Corr. Coeff.
Dilution 1:50
RSD [n = 10, %]
Dilution 1:50
Recovery [%]
1 Chloride 1.423 0.99996 2.18 101.1
2 Nitrate 2.128 0.99992 1.88 102.5
3 Sulfate 1.536 0.99995 1.42 100.6

This information has been sourced, reviewed and adapted from materials provided by Metrohm AG.

For more information on this source, please visit Metrohm AG.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Metrohm USA Inc.. (2020, May 01). Automatic Calibration of Analytes Using a Combination of Inline Dilution and Subsequent Inline Ultrafiltration. AZoM. Retrieved on July 21, 2024 from https://www.azom.com/article.aspx?ArticleID=15446.

  • MLA

    Metrohm USA Inc.. "Automatic Calibration of Analytes Using a Combination of Inline Dilution and Subsequent Inline Ultrafiltration". AZoM. 21 July 2024. <https://www.azom.com/article.aspx?ArticleID=15446>.

  • Chicago

    Metrohm USA Inc.. "Automatic Calibration of Analytes Using a Combination of Inline Dilution and Subsequent Inline Ultrafiltration". AZoM. https://www.azom.com/article.aspx?ArticleID=15446. (accessed July 21, 2024).

  • Harvard

    Metrohm USA Inc.. 2020. Automatic Calibration of Analytes Using a Combination of Inline Dilution and Subsequent Inline Ultrafiltration. AZoM, viewed 21 July 2024, https://www.azom.com/article.aspx?ArticleID=15446.

Ask A Question

Do you have a question you'd like to ask regarding this article?

Leave your feedback
Your comment type
Submit

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.