Metrohm is continually developing its own range of columns and is enhancing existing separation column types using advanced materials and manufacturing processes. The result is columns with exceptional separation performance, high stability, short retention times and lower costs per injection.
The high-efficiency anion separation column Metrosep A Supp 5 is supplied as a 2 mm iColumn. The «i» stands for «intelligent»: A single chip stores the serial number of the column as well as all of the functional parameters such as maximum permissible flow and maximum permissible pressure.
Furthermore, data such as the number of determinations and working hours are recorded automatically and provided to the IC System for monitoring the correct function of the separate system components.
The anion separation column comes in two lengths of 150 and 250 mm, each with a 2.0 mm inner diameter. It is perfectly suited for the separation of standard ions with an economical eluent flow.
Effect of column length on resolution and the analysis times under standard conditions using conductivity detection and chemical suppression:
Eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 10 µL; flow rate: 0.18 mL/min.
1 2 mg/L fluoride
2 3 mg/L chloride
3 5 mg/L nitrite
4 10 mg/L bromide
5 10 mg/L nitrate
6 10 mg/L phosphate
7 10 mg/L sulfate
Applications
The Metrosep A Supp 5 is ideally suited for routine analysis of the seven standard anions and divalent and monovalent carboxylic acids. Applications can be found in all industries: in the pharmaceuticals and food industries, for quality control, in forensic analysis, for water and environmental analysis and in process analysis.
Chlorite and bromate in drinking water
Column: Metrosep A Supp 5 - 250/2.0; eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 5 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.
Multi-component analysis
Column: Metrosep A Supp 5 - 250/2.0; eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 10 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.
Analysis of explosion residues
Column: Metrosep A Supp 5 - 250/2.0; eluent with gradient: eluent A: 2% acetone in ultrapure water, eluent B: 20% acetone, 32 mmol/L sodium carbonate, 10 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 5 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.
Polyethylene pellets with Combustion IC
Column: Metrosep A Supp 5 - 150/2.0; eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 10 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.
The Metrosep A Supp 5 in 2 mm displays ideal peak shapes. Free selection of the column length enables high flexibility according to application.
Technical Information
| . |
. |
| Housing |
PEEK |
| Substrate |
Polyvinyl alcohol with quaternary ammonium groups |
| Standard eluent |
3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate |
| Standard flow |
0.18 mL/min |
| Maximum flow |
0.21 mL/min |
| Maximum pressure |
20 MPa |
| Particle size |
5 µm |
| Standard temperature |
25 °C |
| Temperature range |
20–60 °C |
| pH range |
3–12 |
| Organic modifier (eluent) |
0–100% acetone, acetonitrile and methanol |
| Organic modifier (sample) |
0–100% acetone, acetonitrile and methanol |
| Storage |
in standard eluent |
| Capacity |
18 µmol Cl- (150 × 2 mm)
27 µmol Cl- (250 × 2 mm) |
