Metrosep A Supp 5 - Microbore: 2 mm Anion Column for Ion Chromatography

Metrohm is continually developing its own range of columns and is enhancing existing separation column types using advanced materials and manufacturing processes. The result is columns with exceptional separation performance, high stability, short retention times and lower costs per injection.

The high-efficiency anion separation column Metrosep A Supp 5 is supplied as a 2 mm iColumn. The «i» stands for «intelligent»: A single chip stores the serial number of the column as well as all of the functional parameters such as maximum permissible flow and maximum permissible pressure.

Furthermore, data such as the number of determinations and working hours are recorded automatically and provided to the IC System for monitoring the correct function of the separate system components.

The anion separation column comes in two lengths of 150 and 250 mm, each with a 2.0 mm inner diameter. It is perfectly suited for the separation of standard ions with an economical eluent flow.

Effect of column length on resolution and the analysis times under standard conditions using conductivity detection and chemical suppression:

Eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 10 µL; flow rate: 0.18 mL/min.

1 2 mg/L fluoride
2 3 mg/L chloride
3 5 mg/L nitrite
4 10 mg/L bromide
5 10 mg/L nitrate
6 10 mg/L phosphate
7 10 mg/L sulfate

Applications

The Metrosep A Supp 5 is ideally suited for routine analysis of the seven standard anions and divalent and monovalent carboxylic acids. Applications can be found in all industries: in the pharmaceuticals and food industries, for quality control, in forensic analysis, for water and environmental analysis and in process analysis.

Chlorite and bromate in drinking water

Column: Metrosep A Supp 5 - 250/2.0; eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 5 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.

Multi-component analysis

Column: Metrosep A Supp 5 - 250/2.0; eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 10 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.

Analysis of explosion residues

Column: Metrosep A Supp 5 - 250/2.0; eluent with gradient: eluent A: 2% acetone in ultrapure water, eluent B: 20% acetone, 32 mmol/L sodium carbonate, 10 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 5 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.

Polyethylene pellets with Combustion IC

Column: Metrosep A Supp 5 - 150/2.0; eluent: 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate; column temperature: 30 °C; sample volume: 10 µL; flow rate: 0.18 mL/min; conductivity detection with sequential suppression.

The Metrosep A Supp 5 in 2 mm displays ideal peak shapes. Free selection of the column length enables high flexibility according to application.

Technical Information

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Housing PEEK
Substrate Polyvinyl alcohol with quaternary ammonium groups
Standard eluent 3.2 mmol/L sodium carbonate, 1.0 mmol/L sodium hydrogen carbonate
Standard flow 0.18 mL/min
Maximum flow 0.21 mL/min
Maximum pressure 20 MPa
Particle size 5 µm
Standard temperature 25 °C
Temperature range 20–60 °C
pH range 3–12
Organic modifier (eluent) 0–100% acetone, acetonitrile and methanol
Organic modifier (sample) 0–100% acetone, acetonitrile and methanol
Storage in standard eluent
Capacity 18 µmol Cl- (150 × 2 mm)
27 µmol Cl- (250 × 2 mm)

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