Practical LC-MS/MS Method Development

This course offers practical training for the practicing scientists. It will take the participants step-by-step through the concepts and techniques to develop LC-MS/MS methods. The emphasis is on practical issues associated with developing LC-MS/MS methods for small molecules. It also emphasizes problem-solving skills with examples encountered in the pharmaceutical industry and other fields. This course will provide the participants with an updated overview and a solid working knowledge of LC-MS/MS. The participants will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guides, and key applications. After this course, the participants will be able to independently develop their own LC-MS/MS methods. New technologies and techniques, such as monolithic chromatography and hydrophilic interaction liquid chromatography (HILIC) will be presented. Since some of the participants are from the pharmaceutical industry, which is regulated by GLP and GMP, some regulation and validation concepts will be introduced.