Colorimeters are widely used to determine the concentration of different substances such as drugs, food ingredients, textile products, beverages and many chemicals present in a solution.
The working mechanism of a colorimeter is based on the Beer-Lambert’s law, which states that the amount of light absorbed is directly proportional to the concentration of the medium. A colorimeter consists of a light source, filters, or a monochromator to select a specific wavelength, a microprocessor to determine the amount of light absorbed compared to a reference standard, and an output display.
To determine the concentration of an unknown solution, the test solution is poured into a cuvette and placed inside the equipment.
Light of a specific wavelength then passes through the solution, in which the transmitted light is detected and processed by the microprocessor.