Mid-Infrared Rapid Imaging Microscopy System - Spero™

The Spero developed by Daylight Solutions is a purpose-built fully integrated high spatial resolution high throughput Mid Infrared rapid imaging microscopy system. Spero incorporates Quantum Cascade Laser technologies and provides full frame, real-time imaging capabilities previously unavailable with benchtop instrumentation.

Key Features

Some of the key features of Spero include::

  • Real-time chemical imaging
  • Rapid acquisition of full-spectrum hyperspectral datacubes
  • High-brightness, broadly tunable laser source
  • Synchrotron class spatial resolution and sensitivity
  • Specifically designed for mid-infrared spectral imaging analysis of materials research and biomedical samples
  • Reflection and transmission modes of operation
  • Fully automated stage
  • Visible and infrared high N.A objectives
  • Instrument control and data display via Ethernet to PC
  • Turn-key small footprint integrated mid Infrared  chemical imaging incorporating room temperature focal plane array
3D Infrared Microscopy for Preclinical Pathology


The specifications of Spero are given in the table below:

Parameter Sym Units Hi-Mag Lo-Mag Visible
Camera Array Size -- -- 480 x 480 480 x 480 3k x 2k
Image Pixel Size -- µm 1.4 µm 4.3 µm 0.9 µm
Diffraction-Limited Spatial Resolution Δx µm < 5 µm @ v = 1800 cm-1 < 25 µm @ v = 1800 cm-1 < 3 µm @ λ = 0.55 µm
Numerical Aperture NA -- 0.70 0.15 0.13
Wavelength Coverage Δλ µm (cm-1) 5.5 µm – 11.1 µm (900 cm-1 - 1800 cm-1) 0.45 µm - 0.65 µm
Spectral Resolution Δν cm-1 4 cm-1 N/A
Magnification M - 12.5X 4X 4X
Working Distance WD mm > 5 mm > 25 mm > 15 mm
Field of View FOV mm 650 um 2000 um 650 um
Frame Rate -- fps 30 fps 30 fps
3D Histology with the Spero™ Infrared Microscope

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