Automation of Gel Permeation Chromatography – AZURA GPC Cleanup System 10

KNAUER´s new AZURA GPC (Gel Permeation Chromatography) Cleanup system 10 is designed to automate work-intensive clean-up tasks. Users no longer have to waste time and labor with manual sample preparation. The new Cleanup system provides better reproducibility and quality of sample work-up.

Based on the AZURA device platform, it is possible to customize the system to meet individual requirements. The dedicated system processes up to 16 samples, while only requiring a minimal bench space. Mobile Control provides a smart and cost-effective user interface for device control directly at the system.

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Layout of the AZURA GPC Cleanup System

The AZURA GPC Cleanup system can be easily adapted to pre-established laboratory procedures to perform a broad range of GPC sample preparation tasks.

The pre-configured system consists of 16 sample loops, controlled by two 16-port multi-position valves. The GPC tubing guide methodically sorts loop tubings and outlet tubings of fractionation. Sample loops with a volume of either 5 ml A5329-1 or 1 ml A5329-2 are wound-up.

An additional multi-position valve assists by collecting up to 16 fractions. All 16-port multi-position valves and the manual injection valve, are integrated with an Assistant. A variable single wavelength UV detector monitors elution of separated compounds.

In addition to this small detector, a second Assistant surrounds the system pump with a pressure sensor and a valve to either combine or bypass the GPC column into the flow path.

Operating the AZURA GPC Cleanup System

AZURA Mobile Control operates the GPC Cleanup System. This intuitive user interface is operated on a tablet which is directly mounted at the system. It automatically detects devices and enables system configuration. As a result of block programming, techniques are formulated rapidly with minimum number of clicks. The Mobile Control makes sample loop loading easy to manage by synchronously switching both valves at the press of a button.

What is GPC Cleanup?

GPC Cleanup is chiefly used for performing basic clean-up tasks on a broad range of sample matrices, including tissues, plants, foodstuffs, and environmental samples.

The separation of components occurs according to their molecular weight and due to the heterogeneous pore size of the stationary phase. High-molecular substances such as proteins, lipids, cellular components, natural resins, and steroids interfering with ensuing analysis, e.g. of pesticides, are efficiently eliminated.

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Key features

System Layout

  • 16 outlets of fraction collection
  • 16 sample loops with each 1 ml A5329-2 or 5 ml A5329-1
  • Pump with pressure sensor (maximum flow rate of 10 ml/min)
  • GPC tubing guide sorts sample loops and outlet tubings of fraction collection
  • UV detector with variable wavelength
  • 10” touch screen with Mobile Control Chrom directly at the system
  • Electrical and manual valves for controlling the flow path

In accordance with established methods to determine pesticide residues:

  • SW-846 Method 3640A - US Environmental Protection Agency
  • Method 984.21 - AOAC international
  • L 00.00-34 Method in accordance with §64 LFGB, (formerly § 35 LMBG)
  • AEN 12393 and EN 1528 - European Standard

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