The Lyncée Transmission series uses Digital Holographic Microscopy (DHM) to quantitatively image and measure transparent and semi-transparent samples with interferometric resolution. Holograms are collected and digitally rebuilt to extract 3D information, which is then evaluated by specialized software to deliver unique and significant insights for use in life sciences and materials science.
DHM offers label-free imaging in the life sciences and measures morphology and intracellular content (dry mass). These metrics are endogenous indicators of cell health and biological processes, enabling applications in cell viability assessment, dynamic monitoring, and 4D cell tracking.
DHM is used in materials science to assess thickness variation, surface topography, and intra-sample characteristics such as defects and variations in refractive indices. It also monitors 3D position and dynamic changes over time. It can measure optical MEMS, displays, and acoustofluidic devices as it is compatible with the stroboscopic module.
Key Features
Image Credit: Park Systems
Superior DHM Performance
- Millisecond time resolution
- Single-shot full-field imaging
- Up to 100 kfps acquisition rate
Address All Experimental Needs
- Several options tailored to specific applications
- Multi-objective, multi-wavelength
- Fully upgradeable
Lyncée Transmission Series: Workflow
Image Credit: Park Systems
In a single shot at camera speed, data acquisition encodes all of the sample’s information in the recorded hologram. Then, a real-time numerical reconstruction of the recorded hologram is performed. Refocusing the phase image after image acquisition and obtaining quantitative information that sheds light on the monitored sample are made possible by numerical reconstruction.
Transmission DHM Models
Image Credit: Park Systems
The Lyncée Transmission series (Lyncée T1, T2) includes single- and twin-laser source combinations designed for accurate optical topography. Each model is an appropriate instrument for its intended use: Lyncée T1 for most applications, and Lyncée T2 for large-step-height samples.
Objective Lens Sets
Image Credit: Park Systems
The objective turret could accommodate up to six objectives, with magnifications ranging from 1.25× to 100× (air and oil immersion). This enables fast switching between wide-field overview and high-magnification examination.
Motorized Stage
Image Credit: Park Systems
The optional motorized stage permits automatic acquisitions at predetermined XYZ points, allowing many sites to be recorded without human interaction. The motorized stage also enables the stitching of numerous acquisitions, resulting in a broader field of view for investigating large samples. In the biological sciences, it can be used with standard multi-well microplates (up to 384 wells) for high-content and high-throughput screening.
Fluorescence Module
Image Credit: Park Systems
When paired with the Lyncée Transmission series, the optional fluorescence module enables simultaneous quantitative phase imaging and epifluorescence observation with the same objective lens. This enables direct connection between label-free DHM measurements and fluorescence reference assessments in applications such as cardiotoxicity testing and single-cell analysis. The plug-and-play module allows numerous light sources and cameras, and specific filters prevent crosstalk.
Environmental Chamber
Image Credit: Park Systems
The ideal addition to non-perturbing multi-day time-lapse research. It provides precise control over temperature, O2, CO2, and humidity, comparable to a complete incubator. An optional gas mixer allows for O2 control in hypoxia and hyperoxia investigations.
Principle of Digital Holographic Microscopy
DHM Technology
Image Credit: Park Systems
For life science applications, the Lyncée Transmission series offers real-time Quantitative Phase Imaging (QPI) of living cells. Segmentation then offers biological characteristics for individual cells, such as viability, confluency, and cell dynamics.
Applications
Micro-Optics
Image Credit: Park Systems
Micro-Fluidics and PIV
Image Credit: Park Systems
Acoustofluidics
Image Credit: Park Systems
Displays
Image Credit: Park Systems
Lipid Droplet Quantification
Image Credit: Park Systems
Cell Dynamics
Image Credit: Park Systems
Correlation between DHM and Fluorescence
Image Credit: Park Systems
Cell Migration Screening
Image Credit: Park Systems
Softwares
Koala Acquisition and Analysis Software
Image Credit: Park Systems
Koala provides a real-time, user-friendly interface with GPU-accelerated performance and flexible export of images and analytical data, making it the key platform for DHM hardware control, image capture, processing, and data visualization.
Cell Analysis Tool (CAT) Software for Bioanalysis
Image Credit: Park Systems
CAT is an analysis software created exclusively for life sciences experiments. It integrates with the proprietary acquisition software Koala to give a comprehensive workflow from image acquisition to data visualization and report production.