A-TEEM™ is a new molecular fingerprinting technique with many advantages. All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications. The A-TEEM acquisition and analysis timeframe is on the order of seconds to minutes thus effectively competing with other techniques and procedures that may take hours to weeks! A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.
Dr. Adam Gilmore
Adam Gilmore, PhD, is a Fluorescence Product Manager at Horiba Instruments Inc. He received his Ph.D. in 1992 from the University of Hawaii at Manoa in the Department of Plant Molecular Physiology. His dissertation centered on the first simultaneous absorbance and fluorescence measurements to define a causal relationship between xanthophyll-cycle carotenoids and photoprotective nonradiative dissipation of excess energy in photosystem II, measured by chlorophyll fluorescence. He pursued the physiological significance of this subject during his first postdoctoral tenure at the Carnegie Institution of Washington’s Department of Plant Biology and its biophysical mechanism during his second postdoctoral tenure at the University of Illinois at Urbana Champaign’s Laboratory for Fluorescence Dynamics. He worked for seven years at the Australian National University as a Research Fellow and Fellow followed by a one year sabbatical at UC Berkeley’s LNL before joining Horiba in 2004. At Horiba he has developed and patented application-specific instruments and software methods with primary foci on nanotechnology, water treatment monitoring, and molecular fingerprinting applications. The most significant developments have included simultaneous absorbance-fluorescence instruments utilizing multichannel detectors.