Applications of Nanostructured Hydroxyapatite for Osteogenesis and Angiogenesis

Hydroxyapatite is a calcium phosphate ceramic which is very similar to the mineral component of bone.

It has excellent biocompatibility and when it is implanted in the body, it has the capacity to form strong and stable chemical bonds with bone tissue. Microscale Hydroxyapatite (HA) has been used extensively in the healthcare industry in prosthetic coatings and bone graft substitutes.

With nanotechnology emerging, nanoscale HA has been studied to improve HA properties. Nanostructured materials can imitate surface characteristics present in natural tissues improving their biological performance.

nanoXIM - Nanocrystalline HA Powders

FLUIDINOVA developed the nanoXIM HAp200 product series, which are high purity, single-phase, nanocrystalline HA powders. These products are well suited for medical applications as they present chemical and structural similarity with natural bone.

The nanoXIM HAp200 series has a high specific surface area greater than 100 m2/g and an accurate stoichiometry of calcium or phosphate ions resulting in a Ca/P ratio always near to 1.67.

Impact of nanoXIM HAp202 in a Co-Culture System with Endothelial and Mesenchymal Stem Cells

After implantation of bone graft, the formation of a rapid and stable microvasculature is required to ensure the metabolic requirement of recruited cells to begin the bone regeneration process and support the recently formed tissue.

The process includes a tight communication between endothelial cells, involved in the creation of a vascular network, and mesenchymal stem cells, which can differentiate into osteogenic cells. On implanting of a biomaterial, an adequate environment must be guaranteed for all cell types involved in regeneration of bone.

For that reason, it was investigated whether a three-dimensional substrate produced with nanoXIM HAp202 could support a co- culture of human dermal microvascular endothelial cells (HDMECs) and human mesenchymal stem cells (HMSCs). [Laranjeira M, et al, 1]

It is seen that nanoXIM HAp202 substrates provide an adequate microenvironment for cell viability and proliferation for both monocultures and co-cultures as shown in Figure 1.

Figure 1. Cell viability and proliferation of monocultured and co-cultured HDMECs and HMSCs assessed by DNA quantification (A) and MTT assay (B) for 7, 14 and 21 days of culture. Data kindly provided by Marta Laranjeira.

The alizarin red staining showed that both co-cultures and monocultures stained positive for calcium deposits showing induction in mineralized tissue formation as seen in Figure 2.

Figure 2. Alizarin red histochemical staining performed in nanoXIM HAp202 substrates without cells (control), with HMSCs and co-culture for 7, 14 and 21 days. Data kindly provided by Marta Laranjeira

With regards to the genetic profile of HDMECs and HMSCs in monocultures and co-cultures, the expression of angiogenesis and osteogenesis related genes were observed. Furthermore it becomes very essential to note that HDMECs could proliferate in 3D nanostructured surfaces, as against previous reports that cannot adhere and proliferate in microsized HA structures. [Laranjeira M, et al, 1]

Conclusions

It can be concluded that the substrates obtained with nanoXIM HAp200 series could support a co-culture system of endothelial and mesenchymal stem cells and induced important characteristics with regards to angiogenesis and differentiation of HMSCs into the osteoblastic lineage.

HDMECs were capable to proliferate on nanoscale HA, which does not take place in nanoXIM HAp200  products are promising materials that can be used in bone regeneration and tissue engineering applications. One approach that the material must be pre-seeded with mesenchymal and endothelial cells before implantation, for a more rapid bone regeneration process.

Reference

  1. Laranjeira M, Fernandes M, Monteiro F. Response of Monocultured and Co-Cultured Human Microvascular Endothelial Cells and Mesenchymal Stem Cells to Macroporous Granules of Nanostructured-Hydroxyapatite Agglomerates. Journal of biomedical nanotechnology. 2013;9(9):1594-606.

About FLUIDINOVA

Fluidinova is a high technology engineering manufacturer company specialized in high quality and high purity nano Hydroxyapatite as a raw- material for medical, cosmetics or other applications, in both paste and powder forms.

Fluidinova provides directly to its clients worldwide, willing to improve their current range of products or develop new ones through R&D of innovative products or processes in partnership with national and international companies.

This information has been sourced, reviewed and adapted from materials provided by FLUIDINOVA.

For more information on this source, please visit FLUIDINOVA.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Fluidinova. (2018, August 06). Applications of Nanostructured Hydroxyapatite for Osteogenesis and Angiogenesis. AZoM. Retrieved on October 13, 2024 from https://www.azom.com/article.aspx?ArticleID=10945.

  • MLA

    Fluidinova. "Applications of Nanostructured Hydroxyapatite for Osteogenesis and Angiogenesis". AZoM. 13 October 2024. <https://www.azom.com/article.aspx?ArticleID=10945>.

  • Chicago

    Fluidinova. "Applications of Nanostructured Hydroxyapatite for Osteogenesis and Angiogenesis". AZoM. https://www.azom.com/article.aspx?ArticleID=10945. (accessed October 13, 2024).

  • Harvard

    Fluidinova. 2018. Applications of Nanostructured Hydroxyapatite for Osteogenesis and Angiogenesis. AZoM, viewed 13 October 2024, https://www.azom.com/article.aspx?ArticleID=10945.

Tell Us What You Think

Do you have a review, update or anything you would like to add to this article?

Leave your feedback
Your comment type
Submit

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.