Determination of Nicotine in Tobacco by Non-Aqueous Titration

Nicotine is a nitrogenous alkaloid that stimulates the parasympathetic nerves. It is highly addictive and is hazardous to health. The determination of the amount of nicotine in tobacco products is therefore important.

This Application Note describes a simple method to determine this chemical in tobacco using non-aqueous titration. For comparison, the results determined by GC and IC on the same sample are given.


Chesterfield original cigarette tobacco

Sample Preparation

For the sample, 6 g tobacco is placed in a 500 mL Schott flask into which 2 g barium hydroxide octahydrate is then placed and stirred. Following this, 30 mL saturated barium hydroxide solution is added and then 200 mL extraction solvent. This solution is then placed for 20 min in an ultrasonic bath. At this point the organic phase is collected in an Erlenmeyer flask and placed over anhydrous magnesium sulfate for drying. The suspension is filtered and the filtrate collected.




Blank and Sample Determination

25 mL of the filtrate is placed into a 100 mL beaker and made up to 60 mL with ethanol. The solution is titrated with c(HClO4) = 0.1 mol/L in acetic acid until the first equivalence point is passed.



This information has been sourced, reviewed and adapted from materials provided by Metrohm AG.

For more information on this source, please visit Metrohm AG.


Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Metrohm USA Inc.. (2023, May 29). Determination of Nicotine in Tobacco by Non-Aqueous Titration. AZoM. Retrieved on April 19, 2024 from

  • MLA

    Metrohm USA Inc.. "Determination of Nicotine in Tobacco by Non-Aqueous Titration". AZoM. 19 April 2024. <>.

  • Chicago

    Metrohm USA Inc.. "Determination of Nicotine in Tobacco by Non-Aqueous Titration". AZoM. (accessed April 19, 2024).

  • Harvard

    Metrohm USA Inc.. 2023. Determination of Nicotine in Tobacco by Non-Aqueous Titration. AZoM, viewed 19 April 2024,

Ask A Question

Do you have a question you'd like to ask regarding this article?

Leave your feedback
Your comment type

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.