An Introduction to OMNISEC RESOLVE Multi-Detector GPC/SEC System

OMNISEC is a top of the range multi-detector gel-permeation/size-exclusion chromatography (GPC/SEC) system from Malvern Panalytical. It is a total solution for molecular characterization of natural polymers, synthetic polymers, proteins and polysaccharides.

OMNISEC RESOLVE is an integrated, advanced separations module. It has a completely integrated degasser, column oven, pump and autosampler for mobile phase delivery, separation and sample presentation before measurement.

When used along with OMNISEC REVEAL, the pair form a complete GPC/SEC system. This article talks about the benefits and features of OMNISEC RESOLVE. There is also a discussion of the value of OMNISEC as a complete system compared to upgrading an existing system to sophisticated detection.




OMNISEC RESOLVE is a comprehensive separations module available with OMNISEC REVEAL to form a complete system.


The system includes a low pulsation pump which delivers solvent to the detectors and column and has been optimized to enhance the separation quality and baseline stability offering higher quality data and better separations.

It includes automatic back-flushing of the pump seals to minimize chances of salt precipitation in buffers with high salt levels. This makes the system suited for separations in DMF with LiCl or aqueous buffers.


The degasser has an effective low-volume design, which implies that mobile phases can be rapidly exchanged for quick solvent changeover and reduced downtime.

Column Oven

It is possible for the column oven to hold up to six analytical columns on one GE Tricorn 300/10 column. Temperature control up to 65 °C maintains a stable separation temperature increasing measurement sensitivity.


The autosampler is capable of injecting up to two 48 vial trays or 96-well micro-titer plates of samples. The sample compartment has a temperature control feature meaning that polymer samples can be warmed to reduce solvent viscosity for loading or to aid dissolution.

It is also possible to cool protein samples to 4°C to protect them from any degradation or aggregation while awaiting injection. Sample injections up to 100 µL can be done in “microliter pickup mode” without wasting sample since injection reduces the sample need for precious samples such as proteins.

Table 1. Features and benefits of RESOLVE.

Number Feature Benefit
1 Temperature controlled autosampler (4-60°C) Protect fragile samples such as proteins from degradation; reduce polymer solution viscosity for more accurate injection volumes; reduce viscosity of viscous solvents such as DMSO
2 Zero overhead autosampler The autosampler is capable of making injections with no sample overhead to minimize the use of precious sample
3 Low pulsation pump Maximum baseline stability on detectors, especially, the RI and IV
4 Low volume degasser Faster solvent or buffer changeover
5 Injections from vial or 96-well micro-titer plates Inject samples from your preferred format
6 Integrated column oven Consolidates the entire separation in one unit, improves chromatography and separation
7 Temperature controlled column oven (20-65°C) Maintains stable separation temperature, maximizing measurement sensitivity

Benefits of a Complete System

  • The purchase of a new technology is converted into a complete ready-to-use solution. The modules, the OMNISEC RESOLVE and OMNISEC REVEAL, have been developed to work as closely as possible. Combining the two modules makes sure that all detectors work at high precision and sensitivity.
  • Purchase of a total system implies that a single software package is used for controlling the entire system, from data acquisition to analysis. With a complete system there is just one point of contact for performance verifications or repairs. There is no need for any confusion between manufacturers while trying to figure out the root cause of a problem.
  • By providing advanced detection to a system may mean that you can stop using it for previous applications. If there are different requirements for the samples or the GPC/SEC system can be used in different ways, then it may be possible to purchase a new dedicated system for advanced detection.

Arguments for a Detector Alone

In certain cases, purchasing a complete system may be suitable, in others an existing system may just need to be upgraded by adding OMNISEC REVEAL. In case budget is a concern, one can easily connect the OMNISEC REVEAL to existing GPC/SEC system.

This transforms it instantly from a traditional chromatography system to an advanced multi-detection system while minimizing cost. It is possible that there is a dedicated GPC/SEC system where the application has been fully optimized.

Instead of redeveloping and re-optimizing the application on a new system, it might be a cleaner solution to just add the detector module and allow all of the rest of the setup to remain in place.

Table 2. The benefits of a complete system vs the addition of an advanced detector module.

Complete system Module only
A complete ready-to-use solution from a single supplier built to work together Save budget by simply upgrading to multi-detection
Single software package for acquisition and control Use the system that is already running for an established application
Single supplier means any service issues are covered by them with no risk of arguments about the location of the issue and the responsible party  


The detection capability of OMNISEC REVEAL makes it a highly sophisticated GPC/SEC detector and is offered separately or with the OMNISEC RESOLVE, an integrated solvent and sample delivery system.

Separately it can be used to upgrade an existing GPC/SEC system, however,by adding the OMNISEC RESOLVE to create a complete system, there are more advantages, which have been discussed.

It is possible to realize additional benefits. The system is a ready-to-use solution for GPC/SEC comprising everything needed for smooth functioning.

Finally, a single software package is used for support and a single service organization will provide support in case there is a need.

This information has been sourced, reviewed and adapted from materials provided by Malvern Panalytical.

For more information on this source, please visit Malvern Panalytical.


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