Infiltration preparation of samples for electron microscopy can be done using the PELCO® R1 Rotary Mixer. It is a single speed mixer with a slow rotational speed that ensures improved infiltration without air bubbles and is well suited for preparation of tissue samples.
The mixer consists of a cabinet that houses the motor and a Mixer Head that holds the specimen containers. It comes with the Collet Assembly already in position. Only the mixer head needs to be attached to the collet with the supplied black finger screw and locking washer.
Assembly of PELCO® R1 Rotary Mixer
If the Collet Assembly comes loose or is removed: The collet should be attached back to the motor shaft using the set screw supplied and a 1/16” Allen (hex) wrench. The collet should be placed about 0.8 mm (1/32”) from the cabinet so that it does not rub against it during rotation of the mixer head. Another crucial point is to ensure that the set screw strikes the flat portion of the motor shaft during the tightening procedure. Then the mixer head is slipped over the collet. The finger screw is then inserted with the locking washer through a small hole in the mixing head and the finger screw is tightened. Hand tightening is sufficient as the internal tooth washer will prevent slippage between the finger screw and washer head.
The mixer head of the 1055 and 1055-220 mixers utilize the PELCO® Glass Snap-Cap Vials Product Nos. 12701–12708. The "Snap-Cap" feature seals the contents of the vial, protecting both the sample and the operator. An optional rotator head available for this unit with a 23-hole capacity is Prod. No. 1056. Sample vials that fit this optional head are Prod. No. 410 and 411.
After switching on the instrument, a rotation speed of about 10 rpm is obtained. At full load, a high torque motor maintains instrument speed.
Based on which feet the equipment is resting on, a 55° or 35° tilt is obtained.
Connection to an electrical source 110-120 V AC, 60 Hz (No. 1055), or 220-240 V AC, 50/60 Hz (No. 1055-220) is via a standard grounded electrical cord and plug. The motor circuit is protected by two 5 x 20 mm GDB 250 V (0.1 A) fuses.
If the head fails to rotate, the following should be checked:
- The connection of the electrical cord.
- The fuse at the rear of the cabinet.
- The tightness of the collet screw.
Product and accessory descriptions
||PELCO R1 Rotator w/1058 Head, 115 V, 60 Hz
||PELCO R1 Rotator w/1058 Head, 220 V, 50/60 Hz
||R1 Rotator Head with 12 holes, (32 mm O.D.)
||R1 Rotator Head with 23 holes, (16.3 mm O.D.)
||Glass Snap-Cap vials, 4 ml, 23 mm D x 27 mm H
||Glass Snap-Cap vials, 8 ml, 22 mm D x 39 mm H
||Glass Snap-Cap vials, 12 ml, 22 mm D x 51 mm H
||Glass Snap-Cap vials, 16 ml, 30 mm D x 40 mm H
||Glass Snap-Cap vials, 24 ml, 30 mm D x 52 mm H
||Glass Snap-Cap vials, 32 ml, 30 mm D x 63 mm H
||Sample Vials, 2 ml, 12 mm D x 38 mm, cs/288
||Sample Vials, 4 ml, 15 mm D x 48 mm, cs/144
This information has been sourced, reviewed and adapted from materials provided by Ted Pella, Inc.
For more information on this source, please visit Ted Pella, Inc.