The sizing features of NanoBrook Omni are listed below:
- Fast and accurate nanoparticle size distributions
- Compact bench top unit, USB connection
- Multimodal and unimodal size distribution software
- Range: > 0.3 nm to 10 μm
- Dynamic light scattering at 173° and 90°
- Temperature control, -5 °C to 110 °C
- High power 35 mW diode laser
- Three measurement angles: 15°, 90°, and 173°
- Molecular weight determination (relative and absolute via Debye plot)
- ISO 13321 and ISO 22412 compliant results
- Zeta potential for the tough cases
- For zeta potential in organic solvents
- For RNA, peptides, proteins, mAb, and other biological samples
- For high-salt suspensions
- For samples near the I.E.P
- For viscous or oily media
- 1,000 times more sensitive than other methods
- Disposable cuvettes, no alignment or contamination
- Integrated automatic procedures and parameters (SOP)
Rapid, Reliable and Accurate Analysis
The latest NanoBrook Omni particle size and zeta potential analyzer incorporates everything the user may need for rapid, standard, sub-micron measurements of size and zeta potential. Grounded on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and on Doppler velocimetry (electrophoretic light scattering, ELS) for zeta potential, a majority of measurements take just one or two minutes. The instrument also comprises of Phase Analysis Light Scattering (PALS) measurements for samples with low mobilities (saline, PBS).
Three Scattering Angles
Measurements of traditional colloids are typically made at 90° scattering angle due to the unbiased results measured. For proteins and nanoparticles, IgG and peptides, these < 50 nm samples can be computed using the backscattering angle (173°) for ideal S/N and reproducibility of measurements. Lastly, the 15° detection angle can be chosen for increased sensitivity with aggregation measurements. Zeta potential measurements are always performed using the 15° detection angle to reduce diffusion broadening.
Principles of Operation – Sizing
Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using ideal wetting and/or dispersing agents, if needed. A small ultrasonicator is occasionally beneficial in breaking up loosely-held agglomerates. At 173 sample volume may be decreased to 50 μL with a polystyrene, U-shaped, disposable cuvette and the sample is recoverable. A 90° square polystyrene or glass cells (two or three mL) are utilized, one as small as 10 μL (non-disposable). Furthermore, disposable, glass round cells with reusable Teflon stoppers are employed for aggressive solvent suspensions. In every case, only a few minutes are necessary for the sample and cell to equilibrate with the actively regulated temperature setting inside the NanoBrook Omni.
Principles of Operation – Zeta Potential
The NanoBrook Omni utilizes phase analysis light scattering to establish the electrophoretic mobility of charged, colloidal suspensions. In contrast to its cousin, Laser Doppler Velocimetry (LDV, [occasionally referred to as Laser Doppler Electrophoresis (LDE)), the PALS method does not need the application of large fields which may cause thermal issues or denaturation, because in the measurement of phase shift, the particles have to only move a fraction of their own diameter to produce favorable results. In salt concentrations up to 2 molar and with electric fields as small as 1 or 2 V/cm sufficient movement is triggered to obtain superb results. Furthermore, the Autotracking feature makes up for thermal drift.
||Sizing: nano particle and colloidal-sized materials, in any non-absorbing liquid.
Zeta potential: proteins, nano particle, polymer and colloidal-sized materials, suspended in any non-absorb¬ing liquid, with relative permittivity (dielectric constant) > 1.5 and viscosity < 30cP.
||Sizing: > 0.3 nm to 10 μm diameter, depending on refractive index and concentration Zeta potential: 1 nm to 100 μm, sample dependent
||10-11 to 10-7 m2 /V*s
|Zeta potential range
||-500 mV to 500 mV, sample dependent
|Maximum sample conductivity
Zeta potential: 30 mS/cm
||Sizing: 1 to 3 mL disposable plastic, 50 μL disposable, 40 μL quartz flow cell, 10 μL quartz minimum
Zeta potential: 180 μL, 600 μL, 1250 μL
||Sizing: 0.1 ppm to 50 mg/mL, depending on refractive index and concentration
Zeta potential: 40% v/v, sample dependent
||Sizing: Dynamic Light Scattering, DLS
Zeta potential: Electrophoretic & true Phase Analysis Light Scattering, ELS & PALS
||Brookhaven’s TurboCorr, multitau, research grade with 510 channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range.
||Sizing: ± 1% typical
Zeta potential: plus minus 3% typical
||-5 ° C to 110 ° C, ± 0.1 ° C, active control. No external circulator required.
||Purge facility using dry air, nitrogen preferred
||35 mW red diode laser, nominal 640 nm wavelength
||173°, 90° & 15°
||Average & width, lognormal fit, and multimodal size distribution for sizing Doppler Frequency Shift, electro¬phoretic mobility, zeta potential using Smoluchowski, Hückel, or Henry
||ISO13321 and ISO22412 compliant results for sizing
||100/115/220/240 VAC, 50/60 Hz, 150 Watts
||23.3 x 42.7 x 48.1 cm (HWD)
||Temperature 10 ° C to 75 ° C
Humidity 0% to 95%, non-condensing
||Class I laser product, EN 60825-1:2001, CDRH
Particle Sizer and Zeta Potential Analyzer - NanoBrook Omni
Rapid, Reliable, and Accurate Analysis
Principles of Operation - Sizing
Principles of Operation - Zeta Potential