Equipment | Chemical Analysis |Liquid Chromatography

Liquid Chromatography

Liquid chromatography is a chemistry technique that separates proteins, nucleic acids, or molecules in a fluid. In column liquid chromatography, as the fluid passes through a column, constituents within it interact to various degrees with a solid chromatography media or resin. Target molecules are separated according to their interactions, which can be based on molecular size, charge, hydrophobicity, binding interactions or a combination of these factors. After the constituent parts of the liquid pass through the column, they are normally gathered independently, while the concentration of each compound is tracked to generate an elution curve or chromatogram. The mode of detection is based on the target compound. For instance, protein concentration can be observed with a dye-based protein assay. Generally speaking, a UV detector or spectrophotometer is the standard monitoring device.

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