Acquiring Fluorescence-Free Raman Spectra Using Novel Raman Spectrometer

This article describes an innovative Raman spectrometer presented in an handheld format. The use of a temperature-controlled, distributed Bragg reflector diode laser enables the spectrometer to be operated in a sequentially shifted excitation mode. As a result, fluorescence backgrounds, room lights and fixed pattern noise are eliminated and the Raman data are maintained in true spectral space.

Shifted excitation data can be acquired quickly with a shift time penalty of below 2s, thanks to the economical design of the Raman spectrometer. A novel algorithm is used to extract the Raman data from the shifted excitation spectra. The speed of this algorithm is generally three orders of magnitude higher than traditional shifted-excitation algorithms, which operate in spectral space.

This article demonstrates the supremacy of the novel Raman spectrometer and algorithm in terms of signal-to-noise ratio and removal of backgrounds over standard deviation spectra, FT-Raman, shifted excitation Raman difference spectroscopy (SERDS), and traditional multiple-shift excitation techniques.

This information has been sourced, reviewed and adapted from materials provided by Bruker Optics.

For more information on this source, please visit Bruker Optics.

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