Presented by: Parker Lee, Ph.D. and Lavelay Kizekai, Ph.D., Waters Corporation
Presented Live: June 18, 2025
Light scattering techniques are revolutionizing the way we quantify and study nucleic acid (NA) and lipid nanoparticle (LNP) therapies, with important implications for development, RNA quality assessment, and quality control.
This webinar will look at how coupling multi-angle light scattering (MALS) with field-flow fractionation (FFF) or size-exclusion chromatography (SEC) can offer high-resolution data on essential qualities like size distribution, NA integrity, general stability, and payload encapsulation.
Recent developments in SEC column technology have improved the quality and resolution of separations for complicated mixtures of big NA constructs, as well as NA-protein complexes such as Cas9.
FFF-MALS enables gentle, high-resolution separations, making it ideal for evaluating NA delivery systems, such as LNPs, while preserving their integrity.
Dynamic light scattering (DLS) and electrophoretic light scattering (ELS) enable fast, automated batch measurements, allowing for the screening of parameters like size and surface charge.