Presented by: René Gantier, Ph.D. (Repligen)
Presented Live: October 22, 2025
Affinity chromatography has emerged as a highly successful and scalable approach for filtering adeno-associated virus (AAV) particles in gene therapy production. However, using acidic elution conditions (pH 2-3) raises concerns regarding capsid instability and diminished infectivity, even when followed by quick pH neutralization.
This webinar examines the effects of low-pH elution on AAV capsid integrity and biological activity.
The researchers evaluated capsid aggregation and structural stability across three AAV serotypes – AAV5, AAV8, and AAV9 – purified under different elution conditions and incubated at different temperatures, using a combination of size-exclusion chromatography, multi-angle light scattering (SEC-MALS), and functional assays. Cell-based transduction tests are then used to further assess functional infectivity.
The researchers also investigated the downstream impacts of other purification stages, such as tangential flow filtration (TFF), and how they promote viral aggregation. The findings shed light on the durability of AAV particles under acidic settings, highlighting the necessity of modern analytical methods such as SEC-MALS.