Nitrogen and Protein Analysis with the Dumas combustion method

Determining total protein content is fundamental to quality control and protein declaration according to international labeling laws in the food and feed industry as well as research facilities.

Nitrogen and Protein Analysis with Dumas

Image Credit: Elementar Americas Inc.

Protein content is associated with specific product properties and classifications, such as foam formation, dough properties, beer taste, or the differentiation between starch and gluten-free starch.

Highly precise, matrix-independent protein analyses are necessary in all stages of food production. In principle, protein quantification is conducted by a series of specific and non-specific chemical and physical reactions followed by suitable detection.

Full method automation and accordance with industry standards is generally desirable in current lab operations. At present, there are two different widely accepted primary methods for determining total protein content: the high-temperature combustion method according to Dumas and the wet chemical method according to Kjeldahl.

For over a century, the Kjeldahl principle was the most widely utilized method and was described in most standards for determining the total protein content of food products.

However, it is time-consuming, labor-intensive, and involves hazardous and toxic chemicals, which presents issues concerning lab safety and costs. This has led to the Kjeldahl principle being increasingly displaced by the Dumas principle.

Nitrogen and Protein Analysis with Dumas

Image Credit: Elementar Americas Inc.

The Dumas method has significant advantages over Kjeldahl concerning lab safety, labor time, sample throughput, amount of chemical waste, and cost-per-analysis. The high level of automation of the Dumas method also leads to fewer errors.

With Elementar’s next-generation nitrogen and protein analyzers, the cost-per-analysis is drastically reduced compared to “classical” Dumas analyzers.

Nitrogen and Protein Analysis with Dumas

Image Credit: Elementar Americas Inc.

The investment into a rapid N exceed® instrument from Elementar is economically justified even for labs with just ten samples per day. It provides security and confidence in the potential future growth of samples.

Download the Technical Note to learn more about the advantages of the Dumas combustion method for nitrogen and protein determination.

 

This information has been sourced, reviewed and adapted from materials provided by Elementar Americas Inc.

For more information on this source, please visit Elementar Americas Inc.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Elementar Americas Inc.. (2023, September 08). Nitrogen and Protein Analysis with the Dumas combustion method. AZoM. Retrieved on July 12, 2024 from https://www.azom.com/article.aspx?ArticleID=22905.

  • MLA

    Elementar Americas Inc.. "Nitrogen and Protein Analysis with the Dumas combustion method". AZoM. 12 July 2024. <https://www.azom.com/article.aspx?ArticleID=22905>.

  • Chicago

    Elementar Americas Inc.. "Nitrogen and Protein Analysis with the Dumas combustion method". AZoM. https://www.azom.com/article.aspx?ArticleID=22905. (accessed July 12, 2024).

  • Harvard

    Elementar Americas Inc.. 2023. Nitrogen and Protein Analysis with the Dumas combustion method. AZoM, viewed 12 July 2024, https://www.azom.com/article.aspx?ArticleID=22905.

Ask A Question

Do you have a question you'd like to ask regarding this article?

Leave your feedback
Your comment type
Submit

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.