Fluorescence Spectrometers

Fluorescence Spectrometers are used to determine the concentration of a substance in solution using light. It is used when the kind of compound to be reviewed is known. If the compound is not fluorescent when struck by light, it can be made fluorescent by adding a fluorescent label. To perform the test, a beam of light with a specific wavelength is sent through a solution inside a cuvette. The spectrometer then determines the amount of light that is absorbed by the sample and/or the amount emitted by the sample. The concentration of the substance being identified and measured can then be determined because they are directly proportional to the power of the emission.