The DynaPro® NanoStar® from Wyatt Technology is a cuvette-based dynamic light scattering (DLS) device used for studying promiscuous inhibitors, protein solutions, and many types of macromolecules or nanoparticles in solution. To house valuable and scarce samples, quartz cuvettes provide exact robust measurements using a solution measuring just 1µL, while disposable cuvettes provide equivalent sensitivity with a solution measuring just 4µL. A separate, optimized static light scattering (SLS) channel enables true molar mass measurements.
With the aid of the parallel SLS and DLS channels, the NanoStar® establishes size distributions, weight-average molar mass, hydrodynamic radius Rh, and solution properties appropriate to sample stability - the diffusion interaction parameter (kD or D1), the second virial coefficient (A2 or B22), and the temperature of protein melting or aggregation onset (TM, Tonset).
The main features of the DynaPro® NanoStar® are listed below:
- Range of hydrodynamic radius Rh: 0.2-2500nm
- Range of molar mass (could differ with molecular size, composition, or solvent): 300Da-1MDa
- Sensitivity: 0.1mg/mL lysozyme
- Temperature range: -15°C to 150°C
- DYNAMICS® software provides a comprehensive set of analyses for macromolecules and nanoparticles
- Optical fiber pick-up can be integrated with other Wyatt multi-angle light scattering (MALS) detectors such as miniDAWN™ TREOS® or DAWN® HELEOS® II so as to add on-line DLS measurements in the same detection volume as FFF-MALS measurements or chromatographic SEC-MALS