New Polymer-Protein Hybrid Material Opens Path to New Plastic Applications

Researchers at the University of California, Berkeley (USA) have now developed a new strategy for the formation of hybrid materials from synthetic polymers and proteins.

They have thus been able to fuse the specific biological functions of proteins with the advantageous bulk and processing properties of plastics. Polymer-protein hybrid materials may be of use in the manufacture of sensors, nanomachine parts, or drug-delivery systems. As Aaron P. Esser-Kahn and Matthew B. Francis report in the journal Angewandte Chemie, they have successfully synthesized a green-fluorescing biodegradable gel that responds to changes in pH value and temperature.

Previous processes for the production of hybrid materials depended on very specific coupling techniques that could not be used for some protein side-chains. In contrast, the new method developed by the Berkeley researchers is broadly applicable because in principle it is suitable for any protein. The coupling occurs at both ends of the protein chainand these are the same for all proteins: one amino acid group and one carboxylic acid group. Initially, two parallel but mutually independent (orthogonal) reactions are used to activate the two ends of the chain. These are then attached to special chemical anchor points on the polymer. The proteins thus cross-link the individual polymer chains into a three-dimensional network, forming what is known as a hydrogel. A hydrogel is a solid, gelatinous mass consisting of water incorporated in a polymer network. A well-known example of a hydrogel is the soft contact lens.

Francis and Esser-Kahn chose to use a protein that fluoresces green to cross-link their polymer chains. Because the protein maintains its normal folding pattern even after attachment to the polymer, the fluorescence is also maintained: The entire gel fluoresces green.

This hybrid material has a special trait: the cross-linking of the polymer chains is achieved exclusively by means of the proteins. Because proteins can be attacked by proteases, enzymes that disintegrate proteins, these gels are biodegradable. The green fluorescence of the protein is pH-dependent. The gel correspondingly also reacts to changes in pH. It only fluoresces in the basic range; in a lightly acidic medium, the gel no longer fluoresces. Raising the temperature also elicits a response from the gel. The protein denatures at about 70 C, which quenches the fluorescence and causes the gel to shrink.

Posted April 21st,2008

Tell Us What You Think

Do you have a review, update or anything you would like to add to this news story?

Leave your feedback
Your comment type
Submit

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.