May 11 2012
Among the factors that make spiders successful predators is the ingeniously composed and structured material of their fangs.
Predatory success based on a material advantage: Cupiennius salei,
a tropical wandering spider, penetrates the armour of insects of prey
with its venom fangs. The fangs are composed of chitin and proteins
like the prey’s cuticular armour, but their precise chemical composition
and structure are particularly well-adapted to their function.
© R. Barth
Although their armour consists of the same material as their predator's fangs, flies, grasshoppers and other insects that are the usual prey of spiders have little to offer by way of defence against the spider's bite. As researchers of the Max Planck Institute of Colloids and Interfaces in Potsdam, the Max Planck Institute of Microstructure Physics in Halle and the University of Vienna have discovered, the chitin fibres in the fangs of a large Central American wandering spider are arranged and surrounded with proteins in a way that makes the material particularly rigid and thus capable of penetrating its prey's cuticular armour. Thanks to the composition and structure of their materials, the fangs become optimised and reusable hypodermic needles for injecting prey with paralysing venom. The new insights into this biological material could provide inspiration for the optimisation of similar technical materials and devices.
Nature has been using fibre-reinforced materials far longer than technology. The material of the armour deployed by arthropods, such as arachnids, insects and crustaceans, the cuticle, is composed of a multitude of extremely fine layers of chitin fibres. The fibres arrange themselves parallel to the surface and are typically embedded in a protein matrix. This natural composite adapts to its wide-ranging uses through the strong variations in both the composition and form of the material in different parts of the exoskeleton. The arrangement of the fibres and the protein composition have a crucial influence on the mechanical properties of the material.
"In-depth studies of this cuticular armour can provide numerous new ideas for better, bio-inspired material design," says Yael Politi, who has studied the spider at close quarters together with her colleagues. This view is particularly applicable to structures like the fangs as, due to their vital function, their "design" clearly came under particularly strong pressure over the course of evolution. Because the technical perfection of a biological structure can only be completely understood if its natural functions are taken into account, the scientists from the Max Planck Institutes in Potsdam and Halle worked closely with Friedrich Barth, a biologist from the University of Vienna and expert on spiders with particular expertise in their sensory systems and biomechanics.
The arrangement of the chitin fibres influences the properties of the material
In the course of their studies the scientists noticed that the structure of the material in the fangs of the wandering spider Cupiennius salei differs clearly from that in other parts of the skeleton. Specifically, they observed that the chitin fibres are arranged in a particular way there. In a special zone, the fibres in the different layers mainly run in the direction in which high strain trajectories may be expected during biting. This lends the fang tailor-made mechanical resistance, as the chitin fibres are always stiffer parallel to their longitudinal axis than perpendicular to it. "The highest degree of alignment was found in the mid part of the fang", explains Friedrich Barth.
Metal ions increase the stability of the fang tip
The protein matrix in the venom fang is also optimised with a view to its function. Proteins are characterised by high chemical variability and can, therefore, be easily modified. The spider takes advantage of this in its fangs to gain a material-technological advantage over its prey when hunting. "Remarkably, the fang tip and the outer cuticular layers, which are exposed to the highest strain during biting, mainly consist of proteins," says Friedrich Barth. The protein composition changes from the base of the fang to its tip, where the concentration of the amino acid histidine is significantly higher. Histidine is particularly suitable for cross-linking the proteins with metal ions. Because the researchers also discovered zinc and calcium in the protein matrix, they assume that neighbouring fibres in the protein matrix of the fang tip are actually cross-linked. This makes the tip particularly hard and stiff. Moreover, the stable protein matrix also effectively transmits the stress that arises during the penetration of a prey's cuticular armour to the chitin fibres.
The researchers also established that chloride ions are incorporated into the protein matrix. "Interestingly, the chloride ions and each of the metal ions are distributed differently" says Yael Politi. However, the function of the chloride ions, the criteria according to which the distribution of the incorporated elements is selected, and how this affects the mechanical properties of the fangs, remains unclear.
Nonetheless, what is definite is that: "Spider fangs have a highly specialised material structure. The material properties change in fine gradations from the base to the tip of the fang and the external layer of the fangs seems to be resistant to abrasion," explains Yael Politi. An in depth investigation of the different chemical and structural changes enabling the material properties of the cuticular armour to adapt so sensitively to specific biological functions is a highly rewarding objective for the researchers. Their work may well prove to be of practical benefit. "The knowledge we are gaining here could provide the basis for the development of materials for special applications or hypodermic syringes with special shapes and material properties for medical applications," says Politi.
http://www.mpikg.mpg.de/english/cont_issues/news/pm20120511/index.html