An ELISA or Enzyme Linked Immuno-Sorbent Assay enables quick screening and quantitation of analytes against a range of materials. PerkinElmer provides an extensive array of ELISA kits to quantify the safety of dairy, poultry, meat, feed, honey, seafood and grain.
ELISAs involve a minimum of one antibody with specificity for a specific analyte or antigen and constitutes the basic protocol listed below:
- A liquid sample with the target analyte is added to a microwell that is pre-coated with analyte-specific antibodies. Any target analyte in the sample gets attached to the antibodies.
- An enzyme-linked antigen or antibody is added to the microwell, also attaching to the analyte-specific antibodies coated to the microwell.
- A substrate is added which modifies its color as it communicates with the enzymes linked to the antibodies or analytes.
- The color change is quantified with a spectrophotometer.
- Quantitation of the analyte is found by making a comparison of the test sample results with a standard curve of well-known analyte concentrations.