Elsa Cryo-Transfer Holder

The Elsa Cryo-Transfer Holder is the next generation cryo-holder for high-resolution cryo-EM and tomography experiments.


The Elsa™ cryo-transfer holder is considered a next-generation, single-tilt liquid nitrogen holder built for the frost-free transfer of a sample from liquid nitrogen temperature into a transmission electron microscope (TEM). The holder has mainly been utilized for imaging frozen-hydrated, radiation-sensitive specimens for single-particle cryo-electron microscopy (cryo-EM).

  • Larger dewar (redesigned from the ground up): Rises the volume of liquid nitrogen by 2.5X
  • <1.5 nm/minute drift rate: Guarantees that images quality is high at the time of data collection
  • Centrosymmetric design: Decreases settling and drift, at the time of tomography, by retaining the center of gravity during a stage tilt
  • Prolonged hold time: Enables >9 hours below −145 °C and >8 hours of stable, high-resolution imaging
  • Resolve <2.3 Å features: Allows high-resolution imaging even under cryo-conditions

This holder is available with two different tip configurations. The ultra-low profile makes use of the Gatan Quickload™ clipping-free mechanism to safeguard the specimen and offer the utmost tilt range of any side entry cryo-transfer holder accessible currently (±80° tilt). The standard tip, the most practical tip for novice users, utilizes a clipring mechanism to safeguard the sample.

2.7 Å structure of the 20S Proteasome.

2.7 Å structure of the 20S Proteasome. Breakthrough K3 result. A 2.7 Å structure of the 20S Proteasome with the K3 camera and Elsa cryo-holder on a TF20. Data courtesy of Alexander Myasnikov, Michael Braunfeld, Yifan Cheng, and David Agard. Image Credit: Gatan Inc.

Illustrating the loading of a cryo-sample, recovering and drying the Elsa cryo-transfer workstation

Elsa Cryo-Transfer Workstation

Elsa Cryo-Transfer Workstation. Tutorial on how to load a cryo-sample, recover it, as well as dry the Elsa cryo-transfer workstation. Video Credit: Gatan Inc.

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