Separation of Nanoparticles and Macromolecules using MultiFlow FFF – AF2000

The Postnova AF2000 MultiFlow FFF Series is an advanced flow FFF platform designed for separating nanoparticles, proteins, and macromolecules.

Professional Modular Platform

The AF2000 MultiFlow FFF Series integrates the combined technologies and knowledge of 30 years of leadership in FFF. It is the first professional modular Flow FFF platform available on the market, and is fully controlled by the NovaFFF single software platform, which operates the entire system from autosampler to detectors.

The AF2000 MultiFlow platform also has a unique design, which delivers better performance, higher robustness, and more flexibility compared to earlier systems. The technology marks a major breakthrough in FFF science and sets a new benchmark in the area of Flow FFF. As a result, it has become the most sought-after Flow FFF solution for researchers worldwide.

Universal Flexible Separator

Based on the Flow FFF principle, the AF2000 MultiFlow FFF Series uses a cross-flow field as a driving force for the separation. The samples impacted by this field are isolated by their dynamic diffusion on the basis of particle size or molar mass. Different eluents can be used to run the AF2000 at different temperature conditions.

The AF2000 can also use different channel principles and dimensions. The same system can be employed for running circular HollowFiber Flow FFF (HF5) channels or planar Asymmetric Flow FFF (AF4) channels.

The AF2000 MultiFlow FFF Series can be easily used to seperate a wide range of various samples in the field of chemicals, biopharmaceuticals, nanotechnology, environmental, and food-agro-cosmetics.

The MultiFlow FFF is suitable for characterizing proteins, vaccines, antibodies, liposomes, aggregates, synthetic/natural macromolecules, and nanoparticles. The excellent flexibility afforded by the AF2000 MultiFlow FFF allows it to be used as a universal separator for various kinds of analyte systems. It also provides a secure investment option for future project demands and upcoming samples

The AF2000 MultiFlow FFF Series

AF2000 AT - Standard Ambient Temp Flow FFF

AF2000 AT - Standard Ambient Temp Flow FFFThe AF2000 AT is an economical and non-temperature controlled Flow FFF suitable for general level research in companies, universities, and public scientific centers. It comes in three different versions - stainless steel, aqueous metal-free, and multisolvent - and can be operated with a wide range of organic and aqueous solvents.

Circular HollowFiber Flow FFF (HF5) and Planar Asymmetric Flow FFF (AF4) channels can be used simultaneously.

Many detection principles such as inductively coupled plasma mass spectroscopy (ICP-MS), UV-absorbance (UV), multi-angle light scattering (MALS), refractive index (RI), and dynamic light scattering (DLS) can be easily combined.

AF2000 MT - Advanced Mid Temp Flow FFFAF2000 MT - Advanced Mid Temp Flow FFF

The AF2000 MT is a sophisticated, temperature-controlled Flow FFF system that can be used for higher level research in public research institutions and universities as well as commercial-industrial applications and professional research in the field of manufacturing, product development, and quality control.

Just like the AF2000 AT platform, the AF2000 MT is available in stainless steel, aqueous metal-free, and multisolvent versions, and can be operated with many different organic and aqueous solvents. Circular HollowFiber Flow FFF (HF5) and Planar Asymmetric Flow FFF (AF4) channels can be simultaneously used.

Several detection principles such as inductively coupled plasma mass spectroscopy (ICP-MS), UV-absorbance (UV), multi-angle light scattering (MALS), refractive index (RI), and dynamic light scattering (DLS) can be easily integrated. Temperature control is provided for the separation channel in addition to the entire system, including the transfer capillaries existing between the various detectors and modules.

AF2000 HT - Top-level High Temp Flow FFFAF2000 HT - Top-level High Temp Flow FFF

The AF2000 HT is a top-level, temperature-controlled flow FFF system designed for use in research, development and quality control of high temperature macromolecules and polyolefins in public research institutions, universities, and industry.

Available in stainless steel, the AF2000 HT can be operated with organic solvents from ambient to extreme temperature conditions. Ceramics- and stainless steel-based planar Asymmetric Flow FFF (AF4) channels are solely used for these conditions. Various detection principles such as viscometry (Visco) infrared (IR), multi-angle light scattering (MALS), and refractive index (RI) can be used.

Benefits of the AF2000 MultiFlow FFF Series

Broad Separation Range

Other than HTGPC, the separation in HTFFF can carried out for polymers up to molar masses of 10E9 Da. There was no size exclusion effect, which makes HTFFF suitable for separating ultra-large and large polymers as well as nanoparticles.

Gentle Separation Conditions

Separation can be carried out under the absence of stress and shear forces to the macromolecule due to the open channel and the lack of stationary phase. Standard HTGPC issues such as late elution effects, shear induced chain degradation, and filtering/adsorption effects can be prevented.

FOCUS Technology

Incorporating Postnova’s FOCUS technology, the AF2000 HT ensures a stable and continuous detector flow all the time. This is very important to combine RI detectors and flow sensitive viscometers. The Focus technology also offers a higher resolution, higher sample recovery, and better sensitivity.

Another major benefit provided by the Focus technology is that the channel outlet can be linked directly to the detector without using any T-pieces or valves build in between. This helps to ensure low dead volumes, short transfer line, and reduced band broadening.

Cross Flow System

A unique technology is being used by Postnova to produce the cross flow within the FFF channel, depending on a double syringe pump. This pump is regulated in a unique manner so as to prevent pulsation.

The fully inert pump can easily create absolute and continuous flows ranging between 0,005 and 10 ml/min for various solvents. The volumetric-based syringe pump technology allows a specific volume to be removed per time, irrespective of the nature of the temperature and the solvent.

Flexibility and Interfaceability

The AF2000 MT series can be used as a stand-alone system, or alternatively it can be interfaced with current HTGPC systems available from other manufacturers. This provides an added benefit that GPC and FFF can be carried out using the same set of detectors, employing the same temperature chamber for FFF and GPC.

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