DB30: A UV-Vis Spectrophotometer with Double Beam

Edinburgh Analytical’s benchtop double-beam UV-Vis spectrophotometer is designed for analytical, research, and pharmaceutical applications in a compact footprint.

Key Features

  • Fast scanning: Up to 6000 nm/minute to aid sample analysis throughput
  • Optional Visacle® software: For easy PC control
  • Touchscreen interface: Intuitive menus and functions for ease of use for standalone control
  • Automatic accessory recognition: When connected to the DB30
  • Variable bandpass options: 0.5, 1.0, 1.5, 2.0, or 4.0 nm over the range 190–1100 nm
  • Multiple data storage options: USB, SD card, and internal data storage for convenient retrieval of methods and results 

The DB30 is a high-performance UV-Vis spectrophotometer, built with a dual-beam optical system. It records precise and reliable absorbance and transmission measurements across a broad wavelength range, making it an essential tool for analytical tasks that demand the highest levels of accuracy and consistency.

Designed for flexibility and ease of use, the instrument supports a range of measurement formats, including traditional cuvettes, solid samples, and flow-cell setups. Its advanced optical architecture ensures excellent control over stray light, great baseline stability, and precise wavelength and photometric accuracy.

Users can operate the system using an integrated touchscreen or connect it to a PC using Visacle® software. Visacle offers secure data and method management, with an optional upgrade available for CFR Part 11 compliance, supporting data integrity in regulated environments.

Measurement Examples

Core Features

  • UV Absorption Spectra

The DB30 covers the ultraviolet spectrum down to 190 nm, with an absorbance range higher than 3.

This allows for the measurement of samples with high UV absorption, such as the spectra of edible oils illustrated below.

UV absorption spectra of heat-treated extra virgin olive oil, blended oil, virgin olive oil, extra virgin olive oil, and pomace oil; measured in a standard DB30 dual spectrophotometer

Figure 1. UV absorption spectra of heat-treated extra-virgin olive oil, blended oil, virgin olive oil, extra-virgin olive oil, and pomace oil, measured using a standard DB30 dual spectrophotometer. Image Credit: Edinburgh Analytical

Particle Size

  • DB30 NanoSize Package

The DB30 NanoSize package comprises the DB30 primary apparatus, SpectroLink™ technology, and NanoCuvette™ S.

Particle-size determination has become economically accessible through the NanoSize package, with analytical computations performed via SpectroWork™ using advanced machine learning algorithms.

Particle size analysis with the DB30 NanoSize Package. The particle size is calculated from the spectral features in the absorption spectrum

Figure 1. Particle size analysis with the DB30 NanoSize Package. The particle size is calculated from the spectral features in the absorption spectrum. Image Credit: Edinburgh Analytical

Refractive Index

  • DB30 NanoIndex Package

The DB30 NanoIndex package comprises the DB30 primary instrument, SpectroLink™ technology, and NanoCuvette™ One apparatus.

The refractive index of sugar solutions can be readily determined with the NanoIndex package, with subsequent analytical computations performed in SpectroWork™ using advanced machine learning algorithms.

Brix value and refractive index analysis of sucrose solution 12.5 g/100 g water using NanoIndex package

Figure 1. Brix value and refractive index analysis of sucrose solution 12.5 g/100 g water using the NanoIndex package. Image Credit: Edinburgh Analytical

Chromium (VI)

  • Quantitation Made Easy

The DB30 is equipped with an integrated quantitation mode that facilitates concentration analysis. It measures standard solutions and automatically produces a calibration curve.

When analyzing samples of unknown concentration, the DB30 determines the sample concentration directly by referencing the measured absorbance against the previously established calibration curve. This functionality facilitates expeditious, precise, and dependable quantitative analysis while substantially reducing the user effort.

Standard Cr (VI) solutions from 0.0 mg/L to 1.0 mg/L (left to right).

Figure 1. Standard Cr (VI) solutions from 0.0 mg/L to 1.0 mg/L (left to right). Image Credit: Edinburgh Analytical

Cr (VI) standard calibration curve, taken from the DB30 touchscreen interface.

Figure 2. Cr (VI) standard calibration curve, taken from the DB30 touchscreen interface. Image Credit: Edinburgh Analytical

Download the Brochure for More Information

Specifications Table

Source: Edinburgh Analytical

Parameter Description
Optics Czerny-Turner, dual-beam monochromator
Wavelength Range 190–1100 nm
Spectral Bandwidth 0.5 nm, 1 nm, 1.5 nm, 2 nm, and 4 nm
Stray Light ≤0.10% T (220 nm NaI, 340 nm NaNO2)
Wavelength Accuracy ±0.1 nm D2 peak 656.1 nm
Wavelength
Repeatability
±0.1 nm
Photometric Range Absorbance: -3.4 to +3.4, %T: 0 to 300, Concentration: 0,000 to 9,999
Photometric Accuracy ±0.002 Abs (0–0.5 Abs), ±0.004 Abs (0.5–1 Abs), ±0.008 Abs (1–2 Abs) ±0.3% T
Photometric Reproducibility ±0.0003 Abs (0–0.5 Abs), ±0.0005 Abs (0.5–1 Abs), ±0.001 Abs (1–2 Abs)
Wavelength Scan Speed 10, 100, 200, 400, 800, 1200, 2400, 3600, 6000 nm/minute
RMS Noise ≤0.00003 Abs (0 Abs, 500nm)
Baseline Stability <0.0003 Abs/hour (500 nm, two-hour lamp warm-up period)
Baseline Flatness ±0.0009 Abs (200–950 nm)
Light Source Tungsten-halogen and deuterium lamps
Light Source Switching Automatic switching selectable for 325–370 nm range
Detector Silicon photodiode
Display 7” touchscreen
Software Touchscreen operation, PC-controlled software, or CFR-compliant PC-controlled software
Dimensions 500 mm (W) × 475 mm (D) × 250 mm (H)
Net Weight 20 kg (approx)
Power Supply 100–240 V, 50/60 Hz, 150 VA
Ambient Temperature 10–35 °C
Output Device USB flash drive, SD card
Interface USB PC interface

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