Posted in | Physical Properties Testing | Analytical Test Sieves

SorbaDex™ – For the Separation of Biomolecules

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SorbaDex (also known as Cytiva Sephadex) is a beaded composite material made with ultrapure cross-linked dextran. It has good selectivity, excellent resolution, minimal non-specific adsorption, and excellent chemical stability. The effects of buffer and pH on resolution are minimal.

Superior performance, cost-effective replacement for Cytiva Sephadex^™
One of the major advantages of SorbaDex is its ability to quickly remove small molecules and desalt the sample into pure water simultaneously.

SorbaDex is autoclavable at 121 °C, pH 7 for 30 minutes, and is stable in all commonly used buffers.

Source: Sorbent Technologies, Inc.

Grade	Equivalent to Cytiva Sephadex G-25	Equivalent to Cytiva Sephadex G-50
Super Fine	Dry Bead Size: 20 – 50 µm (> 80 %) Water Regain: 2.15 – 2.25 mL/g Swelling: 4 – 6 mL/g MWCO (size exclusion): below 5000 Da Fractionation Range: 1 – 5 kDa (globular proteins) Catalog No.: 801001-01- 100gm	Dry Bead Size: 20 – 50 µm (> 80 %) Water Regain: 4.80 – 5.20 mL/g Swelling: 9 – 11 mL/g MWCO (size exclusion): below 25000 Da Fractionation Range: 1 – 30 kDa (globular proteins) Catalog No.: 801004-01-100gm
Fine	Dry Bead Size: 20 – 80 µm (> 80 %) Water Regain: 2.15 – 2.25 mL/g Swelling: 4 – 6 mL/g MWCO (size exclusion): below 5000 Da Fractionation Range: 1 – 5 kDa (globular proteins) Catalog No.: 801002-01 -100gm	Dry Bead Size: 20 – 80 µm (> 80 %) Water Regain: 4.80 – 5.20 mL/g Swelling: 9 – 11 mL/g MWCO (size exclusion): below 25000 Da Fractionation Range: 1 – 30 kDa (globular proteins) Catalog No.: 801005-01 -100gm
Medium	Dry Bead Size: 50 – 150 µm (> 80%) Water Regain: 2.15 – 2.25 mL/g Swelling: 4 – 6 mL/g MWCO (size exclusion): below 5000 Da Fractionation Range: 1 – 5 kDa (globular proteins) Catalog No.: 801003-01 -100gm	Dry Bead Size: 50 – 150 µm (> 80%) Water Regain: 4.80 – 5.20 mL/g Swelling: 9 – 11 mL/g MWCO (size exclusion): below 25000 Da Fractionation Range: 1 – 30 kDa (globular proteins) Catalog No.: 801006-01 -100gm

Specifications

Source: Sorbent Technologies, Inc.

Catalog No.	Fractionation range (globular proteins) – Mr	pH stability	Bed volume ml/g dry SorbaDex™	Bead size (dry)	Product
801001	1-5 kDa	2 to 13	4 – 6	20-50 µm	SorbaDex™ – 25 superfine
801002	1-5 kDa	2 to 13	4 – 6	20-80 µm	SorbaDex™ – 25 fine
801003	1-5 kDa	2 to 13	4 – 6	50-150 µm	SorbaDex™ – 25 medium
801004	1-30 kDa	2 to 13	9 – 11	20-50 µm	SorbaDex™ – 50 superfine
801005	1-30 kDa	2 to 13	9 – 11	20-80 µm	SorbaDex™ – 50 fine
801006	1-30 kDa	2 to 13	9 – 11	50-150 µm	SorbaDex™ – 50 medium
801007	Hydrated in phosphate buffered saline; pH 7.4, with 0.2% sodium azide				SorbaDex™ – 50 fine
801008					SorbaDex™ – 25 medium

Performance

Source: Sorbent Technologies, Inc.

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High Performance Results Sample: Mouse IgG in PBS Conc.: 12.5 mg/ml Volume: 4500 ml Total IgG: 56.25 grams Buffer: 100 mM Glycine-HCl, pH 2.0 neutralised with 1 M Tris-HCl pH 8.0 System: ÄktaPilot (GE Healthcare) Column: Sorbadex-25 M in BPG 300/500 Diameter: 30 cm Gel Volume: 25 Liters Pressure: 1.0 bar (0.1 mPa or 13 psi) Flow rate during sample loading: 400 ml Flow rate during equilibation and elution: 500 ml Total run time: 63 min
Protein Desalting from 0.8M NaCl IgG (280 nm): dark blue line NaCl (µS): gold line 1 mg IgG anti-Rabbit in 1 mL 0.8 M sodium chloride Water elution
Removal of FITC from IgG IgG (280 nm): dark blue line FITC (550 nm): gold line 1 mg IgG anti-Rabbit and 0.1 µmol FITC in 1 mL DMSO/NaHCO₃ PBS Elution
Oligo Desalting from 0.8M NaCl Oligo (260 nm): dark blue line NaCl (µS): gold line 1 mg oligonucleotide (18-mer) in 1 mL 0.8 M NaCl
Removal of Rhodamine after labeling Oligo (260 nm): dark blue line TAMRA (550 nm): gold line 1 mg oligonucleotide (18-mer) and 0.5 µmol TAMRA in 1 mL DMSO/NaHCO₃
Removal of Ammonia (33%) Dextran Blue (260n m): dark blue line NH₃ (pH): gold line 0.5 mg Dextran Blue (Mr 2,000,000) in 1 mL ammonia (33%) Water elution
Removal of Ammonia (33%) from Oligo Oligo (260 nm): dark blue line NH³ (pH): gold line 1 mg oligonucleotide (18-mer) in 1 mL ammonia (33%) after cleavage