The HORIBA Fluorolog-QM™ series marks the fourth generation of HORIBA's renowned Fluorolog spectrofluorometers, a lineage that began with the original Fluorolog introduced by Spex Industries in 1975.
The Fluorolog-QM embodies decades of HORIBA's expertise as an industry leader in developing and manufacturing spectrofluorometers with unparalleled performance and adaptability.
Featuring meticulously crafted, optically flawless, all-reflective optics, and offering a wide range of light sources, detector options, and sample handling accessories, the Fluorolog-QM delivers the highest sensitivity and greatest versatility available in any spectrofluorometer.
The Fluorolog-QM's capabilities can be expanded to accommodate a diverse range of luminescence experiments through the industry's most comprehensive selection of optional accessories. These enhancements can be integrated after purchase, allowing customers to adapt the system's capabilities and performance as the research needs evolve or funding becomes available.
With thousands of units in operation in universities and research laboratories globally, and cited in tens of thousands of publications, the Fluorolog has established itself as the premier choice for the most demanding research in steady-state, time-resolved, TCSPC, PLQY, and NIR spectroscopy.
Features
Unique Fluorolog-QM™ Benefits
- Utilizes all-reflective optics to ensure ideal performance across the entire spectrum of wavelengths.
- Offers the highest level of guaranteed sensitivity, more than twice that of the Fluorolog-3 model.
- Achieves superior rejection of scattered light through extra-large, coma-corrected monochromators available as a single 350 mm unit or a double additive 700 mm unit.
- Features new software for all steady-state and lifetime experiments, incorporating many novel functionalities.
- Provides an expanded range of measurable light from the deep ultraviolet region to the near-infrared region.
- Allows simultaneous connection and computer control of up to four different light sources and six detectors, offering unparalleled flexibility in the laboratory.
- Includes world-class, user-friendly TCSPC lifetime upgrades capable of speeds up to 100 MHz.
- Enables steady-state and phosphorescence lifetime detection in the near-infrared region up to 5500 nm.
- Allows excitation with deep ultraviolet light down to a wavelength of 180 nm.
Specifications
The specifications listed below apply to the standard Fluorolog-QM-75-22 system.
Additional options and upgrades are available upon request.
Source: HORIBA
| . |
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| Signal-to-Noise Ratio |
(>35,000:1 FSD) |
| Data Acquisition Rate |
1,000,000 points/sec. to 1 point/1000 sec. |
| Inputs |
4 photon counting (TTL); 4 analog (+/- 10 volts); 1 analog reference channel (+/- 10 volts); 2 TTL |
| Outputs |
2 analog (+/- 10 volts); 2 TTL |
| Emission Range with standard PMT |
185 nm to 900 nm (optional to 5500 nm) |
| Light Source |
High efficiency “ECO” friendly continuous 75 W Xenon arc lamp (Optional 450 W Xenon) |
Excitation and Emission
Monochromators |
700 mm, triple grating, coma-aberration corrected, asymmetrical, excitation or emission optimized, Czerny-Turner design with computer controlled slits at entrance, intermediate plane and exit. |
| Slits |
Computer-controlled, continuously adjustable |
| Excitation Grating |
1200 line/mm 300 nm blaze, (Up to two optional gratings can be ordered) |
| Emission Grating |
1200 line/mm 400 nm blaze, (Up to two optional gratings can be ordered) |
| Wavelength Accuracy |
+/- 0.3 nm |
| Minimum Step Size |
0.01 nm (grating dependent) |
| Standard Detection |
Multimode, -20 degrees Celsius cooled, 1600 Volt PMT housing; Multimode: Photon Counting, 3 analog (fast, medium, slow response),
direct and Single-Shot Transient Digitizer (SSTD) mode, and Time-Correlated Single Photon Counting (TCSPC), R928P PMT standard (other PMTs available) |
| R928P PMT Dark Count: |
10 counts per second or less |
| R928P PMT Maximum Linear Count Rate: |
10,000,000 counts per second |
| System Control |
Computer interface with FelixFL spectroscopy software |
| Lifetime Range |
5 ps to seconds with appropriate time-resolved accessories |
Applications
Time-Resolved Electroluminescence with Fluorolog-QM
Image Credit: HORIBA
The highly adaptable Fluorolog-QM allows for the analysis of electroluminescent materials across a wide timescale, spanning from nanoseconds to seconds, all within the integrated system, eliminating the need for external equipment.
Selection Guide for Solid State NIR Detectors on the Fluorolog-QM
Image Credit: HORIBA
Choosing the optimal solid-state detector for a specific set of photoluminescence experiments using the Fluorolog-QM modular research spectrofluorometer.
Photoluminescence Upconversion with the Fluorolog-QM
980 nm DPSS laser mounted to the front of the Fluorolog-QM sample compartment. Image Credit: HORIBA
The latest Fluorolog-QM spectrofluorometers, with their adaptable design, sophisticated software, and universal interface, are perfectly suited for investigating various facets of upconversion phenomena. The Fluorolog-QM-75-21 model analyzes the spectral and time-dependent properties of these materials.
PLQY in NIR with Fluorolog-QM and K-Sphere
Image Credit: HORIBA
When equipped with an integrating sphere, the Fluorolog-QM fluorescence spectrometer is an outstanding tool for measuring Photoluminescence Quantum Yield (PLQY) in the Near-Infrared (NIR) region.
The K-Sphere accessory offers exceptional convenience and ease of use. It directly connects to the sample compartment optics and enables the use of external light sources, such as DPSS lasers, which can be attached to the front of the sample compartment.
The sphere includes easily interchangeable holders for cuvettes, slides, and powder samples. The results demonstrate excellent reproducibility and precision for NIR PLQY measurements across a wide range, spanning nearly two orders of magnitude.
Based on multiple measurements, the standard deviations in the demonstrated PLQY values range from 1.3% for a high Quantum Yield (QY) of 77% to 6.4% for a QY below 1%.
Kinetic Fluorescence Determination of Vitamin B1
Image Credit: HORIBA
Kinetic fluorescence is an analytical method for quantifying non-fluorescent substances. The technique is demonstrated through its application in determining the thiamine concentration (vitamin B1) in a solution.
Monitoring Culture Medium Conditions During Cell Proliferation Using the Veloci BioPharma Analyzer
Image Credit: HORIBA
In the biopharmaceutical industry, closely tracking the conditions of cell culture media is essential for maximizing cell growth. The health of these cells significantly affects the output and quality of Active Pharmaceutical Ingredients (APIs) in areas such as regenerative medicine, monoclonal antibody (mAb) production, protein synthesis, and numerous other applications.
Endogenous Skin Fluorescence in vivo on Human Skin
Image Credit: HORIBA
The fluorescence spectra of naturally occurring protein components that emit light have been extensively studied and utilized to investigate various biological processes.
Fluorescence on Small or Solid Samples
Image Credit: HORIBA
Biological samples can be precious due to the limited availability of starting material or the desire to conserve them. For instance, biological proteins and enzymes are often obtained in small quantities and can be costly.
Recording Fluorescence Quantum Yields
Image Credit: HORIBA
When a fluorophore absorbs a light particle (photon), it enters a higher energy state. The subsequent behavior of this excited molecule varies based on its specific properties and environment, but ultimately, it will lose energy and return to its original, lower energy state.
Photoluminescence Spectroscopy of Quantum Dots
Image Credit: HORIBA
Quantum dots (QDs) show promise in various technological fields, including optoelectronics, biosensing, biolabeling, memory devices, and laser light sources.