Thanks to the introduction of the NanoSight Pro, Malvern Panalytical is able to provide the best-in-class, simple, and rapid nanoparticle tracking analysis (NTA) solution for nano- and biomaterial characterization.
The combination of advanced engineering and a blend of smart features assures NTA measurements are efficient, quick, and accessible to all users. Machine learning-powered NS Xplorer software automates measurements, prevents subjectivity, and provides the highest quality size and concentration data for both light scatter and fluorescence analysis.
Interchangeable lasers ensure application flexibility, whilst Smart Manager connection assures robustness without having to worry about quality issues or downtime.
Image Credit: Malvern Panalytical
Image Credit: Malvern Panalytical
Image Credit: Malvern Panalytical
Overview
The software interface is logical and user friendly, even for a first-time user.
Rebecca Dragovic, Teaching Fellow & Research Scientist, Nuffield Department of Women’s & Reproductive Health, University of Oxford
NanoSight Pro: Expert Nanoparticle Tracking Analysis
Video Credit: Malvern Panalytical
Features and Benefits
Quick Install and Analysis
NanoSight Pro can be used by any user in any lab. The smart installation allows users to get up and running quickly. With minimal setup, simple operation, and intuitive NS Xplorer software, users are able to assess their samples in a matter of minutes.
Intelligent and Automated
NanoSight Pro’s machine learning and intelligent features automate processing and eliminate human error, resulting in superior particle identification and reliable particle tracking for reproducible and high-quality data.
Highest Resolution
The NanoSight Pro identifies nanoscale particles in the range of 10 nm to 1000 nm, as well as low scattering and biological particles. Particle–by–particle analysis ensures concentrated, high-resolution data with visual confirmation.
Power of Fluorescence
In addition to light scatter, fluorescence signals can be detected from fluorescently tagged biologics or synthetic particles, providing even greater insight into samples.
Interchangeable laser modules with four excitation wavelengths can be used in combination with the matching long-pass filters to achieve the optimum setup, which is supported by in-flow analysis for better sampling and tracking of even photobleaching samples.
The greater fluorescence sensitivity ensures subpopulation identification, including surface markers, internal cargo detection, or depreciation of contaminants sensitivity.
What Customers Think
It is the easiest bit of equipment I have ever installed in the lab
Oliver Huseyin, Research Scientist, MIP Diagnostics Limited
Key Applications
The Malvern Panalytical NanoSight Pro has numerous applications, including:
Extracellular Vesicles (EV)
While research into the generation and roles of extracellular vesicles is ongoing, monitoring and controlling their isolation and purification is essential.
The NanoSight NTA allows for quick and easy evaluation of vesicle size and concentration in aqueous buffers, ensuring confidence in the quality of the sample used in any subsequent research, while fluorescence can assist in elucidating the origin subpopulations of EVs.
Virus and Vaccines
When manufacturing vaccines, controlled production processes are essential to ensure that the immune system can appropriately dose and recognize the materials. NTA assists in the optimization of manufacturing processes by ensuring the size distribution of the vaccine is maintained. As NTA is also able to count and size viruses, it can be used as a faster alternative to titer assays.
Drug Delivery and Gene Therapy
With drug delivery and gene therapy products, 70-150 nm is the typical size of particles required to ensure effective disease targeting in the clinical.
NTA is ideally suited to study the size of these particles from early-stage research through to candidate screening, formulation development, and clinical batch monitoring. The concentration measurement allows for dose determination of the final product as well as for use in in vitro and in vivo assays.
Biotherapeutics
Temperature, pH change, agitation, shear, and time can affect the stability of biotherapeutic proteins, causing denaturation and aggregation, which can lead to efficacy loss and the potential for an unwanted immune response. NTA provides high-resolution size distributions of sub-visible aggregates in a formulation for safety and quality assurance.
Nanomaterials/Colloids/Toxicology
Nanomaterials are garnering regulatory attention as they are being incorporated into everyday items. It is essential to fully characterize these particles, their properties, and their contribution to the finished product.
NanoSight can provide a number-based concentration for these nanomaterials and high-resolution size distributions to individuals working at the nanoscale in environmental, industrial, and toxicological domains.
Ultrafine Bubbles
Work on ultrafine bubbles has gained popularity in recent years, with applications ranging from industrial cleaning and water treatment to agriculture and food and medical applications.
Although their relatively low concentration and small size are a challenge for many traditional nanoparticle characterization techniques, NTA is particularly well-suited for the detection and analysis of these bubbles.
Specification
General
Table 1. Source: Malvern Panalytical
| . |
. |
| Technology |
Nanoparticle Tracking Analysis |
| Size range (diameter) 1 |
10 nm - 1000 nm |
| Particle concentration 2 |
106 to 109 particles / mL |
| Minimum sample volume |
250 μL |
| Advanced Concentration Algorithm |
Concentration Upgrade |
System
Table 2. Source: Malvern Panalytical
| . |
. |
| Product Compliance |
Product Laser Class 1 (BS EN 60825-1:2014)
EMC Directive (EN IEC61326-1:2021)
Low Voltage Directive (IEC 61010-1:2010
IEC 61010-1:2010/AMD 1:2016) |
| Camera |
High sensitivity sCMOS (USB-3) |
| Laser information |
Beam wavelength (maximum power output):
405 nm, max power <70 mW
488 nm, max power <55 mW (blue)
532 nm, max power <60 mW (green)
642 nm, max power <50 mW (red) |
| Temperature control range |
5 °C below ambient up to 70 °C |
| Temperature readout |
Automatic |
| Syringe pump |
Continuous sample flow with 1 mL syringes |
| Dimensions (W, D, H) |
34x35x25 cm |
| Weight of instrument |
11 kg |
| Weight of laser module |
1.6 kg |
| Power requirements |
AC 110-240 V, 50-60 Hz, 4.0 A |
| Ambient operating conditions |
Up to 80% rH at 31 °C then decreasing linearly to 50% at 40 °C |
Additional Options
Table 3. Source: Malvern Panalytical
| . |
. |
| Fluorescence – automatic selection 3 |
For up to 5 filters |
1 Dependent on sample and instrument configuration
2 Sample dependent
3 Optional functionality. Long-pass filters available for each laser wavelength
Support
Users can protect their investment and ensure optimal performance at all times with Malvern Panalytical’s service plans.
When a customer purchases a Malvern Panalytical product, this is just the beginning of a long-term working relationship. Malvern Panalytical will support the business based on its needs.
Help Desk
- Operators take responsibility for the call and make sure that the query is answered
- Telephone and email support
Training
Software Downloads
- A simple method for accessing updates and new features
Malvern Panalytical’s Service Options — Choose the Right Plan
Malvern Panalytical Platinum Plan
Ideal for laboratories where instrument uptime is essential to daily performance. The greatest level of responsiveness and “all-inclusive” plan covers any unanticipated repair costs.
Malvern Panalytical Gold Plan
Users can boost laboratory productivity by optimizing instrument efficiency. The company will continue to provide priority response, along with specialized technical and software support, to keep the instrument on track to deliver.
Malvern Panalytical Bronze Visit
Regular preventative maintenance is essential for laboratories looking to improve the performance of their instruments.
Table 4. Source: Malvern Panalytical
| |
Platinum Plan |
Gold Plan |
Bronze Visit |
| Annual PM/PV Test |
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| Telephone / Email Support |
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| Priority Response |
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| Emergency Breakdown visits* |
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| Parts Included |
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| Technical & Software Support |
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| IQ/OQ (Pharma)*** |
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*including labor & travel costs ***available at an additional cost