The InverTau™ platform is a laser scanning confocal unit designed for Fluorescence Lifetime Imaging(FLIM). Fully software-controlled, InverTau is engineered to mount on the side port of an inverted fluorescence microscope. It can be integrated into a custom-built microscope system or purchased as part of a complete system from HORIBA. Versatile solutions are available and supported by local expertise.
Image Credit: HORIBA
Image Credit: HORIBA
Features
- Affordable laser scanning unit for FLIM studies
- Fully computer-controlled confocal optics with intuitive EzTime Image Software for minimized setup time and increased productivity
- Easy transition from confocal laser scanning to widefield imaging with the addition of FLIMera
- Real-time video display at up to 6 fps with InverTau, and up to 30 fps with FLIMera
- Compatible with the side port of an inverted microscope or available as a complete system from HORIBA
- Intuitive software and automated optics streamline setup and enhance workflow efficiency
InverTau
Video Credit: HORIBA
What is Included
- InverTau confocal fluorescence lifetime imaging (FLIM) platform
- FLIMera widefield, video-rate FLIM camera for real-time visualization and lifetime kinetics
- Computer, control electronics, and EzTime Image software included
- Nikon Ti2-U manual inverted fluorescence microscope
- HPPD-720 TCSPC detector (other models available upon request)
- DeltaDiode-C1 controller, CND, and fiber coupler for HORIBA DeltaDiode TCSPC sources (optional)
- Optional HORIBA DeltaDiode lasers (375 to 595 nm) precisely matched for optimal system performance
EzTime Image Software
EzTime Image software is designed with HORIBA’s InverTau, FLIMera, and stage scanning systems. It features an intuitive, automated, touchscreen-friendly interface for controlling TCSPC and MCS, as well as for data acquisition and analysis.
It is a comprehensive application that manages:
- Instrument recognition and control
- Data collection
- Data analysis
Specifications
InverTau Specifications. Source: HORIBA
| |
|
| Modes |
Single point, raster, bi-directional (xy) |
| Scan speed |
Up to 6 fps (bi-directional) at 512x512 |
| Image resolution |
32 x 32 to 4k x 4k |
| Digital zoom |
1 FoV to 0.1 FoV |
| Attachment |
To side port of inverted microscope (C-mount) |
| Control software |
EzTime™ Image software |
| Optical ports |
2 on excitation, 2 on emission |
| Attenuation filters |
Steps from 0-6 OD ND |
| Dichroic filters |
400 nm, 450 nm, 500 nm, 550 nm, 600 nm, 730 SP, 50/50 BS |
| Emission filters |
400BP, 450BP, 500BP, 550BP, 600BP, 440LP, 475LP, 530LP, 570LP, 630LP |
| Pinhole |
10, 25, 50, 100, 200 μm (+ closed and fully open) |
| Photon streaming |
Stream to HDF5 |
| Phasor analysis |
Switchable with histogram |
| Data analysis |
Global up to 5 exponentials |
Key FiPho Electronics Specifications for FLIM. Source: HORIBA
| |
|
| Lifetimes from |
<50 ps |
| Deadtime |
5 ns |
| TCSPC bin width |
<15 ps |
| Histogram Bin Depth |
8, 16 and 32-bit |
| Independent Stops |
1 to 4 |
| Maximum Start Rate |
100 MHz |
| Maximum Rate/Stop Channel |
10 Mcps |
| Operating Mode |
Automatic Forward Timing |
| Streaming Mode |
Photon Streaming (Time-Tag) |
| Acquisition and Analysis Macro Scripting |
Yes |
| PC Interface |
USB 3.0> |
| Software |
EzTime™ Image |